Branching Ionizable Lipids Can Enhance the Stability, Fusogenicity, and Functional Delivery of mRNA

Ionizable lipids with branched tails have been used in lipid nanoparticles (LNPs)-based messenger RNA (mRNA) therapeutics like COVID-19 vaccines. However, due to the limited commercial availability of branched ingredients, a systematic analysis of how the branched tails affect LNP quality has been lacking to date. Herein, a-branched tail lipids are focused, as they can be synthesized from simple commercially available chemicals, and the length of each chain can be independently controlled. Furthermore, symmetry and total carbon number can be used to describe a-branched tails, facilitating the design of a systematic lipid library to elucidate "structure-property-function" relationships. Consequently, a lipid library is developed containing 32 different types of a-branched tails. This library is used to demonstrate that branched chains increase LNP microviscosity and headgroup ionization ability in an acidic environment, which in turn enhances the stability and in vivo efficacy of mRNA-LNPs. Of the branched lipids, CL4F 8-6 LNPs carrying Cas9 mRNA and sgRNA could achieve 54% genome editing and 77% protein reduction with a single dose of 2.5 mg kg(-1). This mechanism-based data on branched lipids is expected to provide insights into rational lipid design and effective gene therapy in the future.

Virtualization of Laboratory Practices Using Visual Basic Excel

The COVID-19 pandemic and its related restrictions forced the reorganization of learning methodology and gave a central role to remote learning. Laboratory experiments are the most affected activity, and several alternatives were described. This work proposes to create calculation tools by simply programming in Visual Basic of Excel to emulate the data acquisition of specific laboratory experiments. The approach appears useful in experiments with a simple setup followed by data analysis. The experiment of gas volumetric properties allows fixing pressure and temperature conditions and measuring the occupied volume. The developed program emulates such operations and reports a computed volume. Further data reduction is the same in both procedures. Such a virtual experience was successfully used with groups of over 100 students. The results obtained were satisfactory compared with those obtained in the laboratory. Detailed analysis of the grades shows that acquired skills are comparable in both methodologies. Consequently, the virtual approach is a flexible option for remote laboratory teaching to complement traditional experimentation. Published 2022 by American Chemical Society and Division of Chemical Education, Inc.

Simple fabrication of an electrospun polystyrene microfiber filter that meets N95 filtering facepiece respirator filtration and breathability standards

During the global spread of COVID‐19, high demand and limited availability of melt‐blown filtration material led to a manufacturing backlog of N95 Filtering Facepiece Respirators (FFRs). This shortfall prompted the search for alternative filter materials that could be quickly mass produced while meeting N95 FFR filtration and breathability performance standards. Here, an unsupported, nonwoven layer of uncharged polystyrene (PS) microfibers was produced via electrospinning that achieves N95 performance standards based on physical parameters (e.g., filter thickness) alone. PS microfibers 3–6 μm in diameter and deposited in an ~5 mm thick filter layer are favorable for use in FFRs, achieving high filtration efficiencies (≥97.5%) and low pressure drops (≤15 mm H2O). The PS microfiber filter demonstrates durability upon disinfection with hydroxyl radicals (•OH), maintaining high filtration efficiencies and low pressure drops over six rounds of disinfection. Additionally, the PS microfibers exhibit antibacterial activity (1‐log removal of E. coli) and can be modified readily through integration of silver nanoparticles (AgNPs) during electrospinning to enhance their activity (≥3‐log removal at 25 wt% AgNP integration). Because of their tunable performance, potential reusability with disinfection, and antimicrobial properties, these electrospun PS microfibers may represent a suitable, alternative filter material for use in N95 FFRs.

The utilization of palm kernel shells and waste plastics in asphaltic mix for sustainable pavement construction. (Special Issue: Technology and innovation for a post COVID recovery and growth: the contribution of industry and academia.)

Natural aggregates are being depleted due to the high demand for road and building construction and need to be replaced with alternative materials. This study investigated the potential of using Palm kernel shells (PaKS) as a partial replacement for natural aggregates (NA) and waste plastics (WP) as a binder. The physical and volumetric properties of the different asphaltic mixes (AM) were assessed using the Marshall Method. The bitumen content of the mix design samples was varied from 4.0% to 7.5% of the total weight of aggregates utilized. According to the Marshall parameters, at 5.5% bitumen content, the maximum Marshall Stability value of the different mix designs increased from 9.8 kN to 12.1 kN and the flow value increased from 3.0 mm to 3.7 mm. The experimental results based on the optimum bitumen content determined by the Marshall method demonstrate that PaKS and WP can be utilized to modify AM. However, additional tests will be needed to evaluate the use of this composition in road construction.

Branching Ionizable Lipids Can Enhance the Stability, Fusogenicity, and Functional Delivery of mRNA

Ionizable lipids with branched tails have been used in lipid nanoparticles (LNPs)-based messenger RNA (mRNA) therapeutics like COVID-19 vaccines. However, due to the limited commercial availability of branched ingredients, a systematic analysis of how the branched tails affect LNP quality has been lacking to date. Herein, alpha-branched tail lipids are focused, as they can be synthesized from simple commercially available chemicals, and the length of each chain can be independently controlled. Furthermore, symmetry and total carbon number can be used to describe alpha-branched tails, facilitating the design of a systematic lipid library to elucidate "structure-property-function" relationships. Consequently, a lipid library is developed containing 32 different types of alpha-branched tails. This library is used to demonstrate that branched chains increase LNP microviscosity and headgroup ionization ability in an acidic environment, which in turn enhances the stability and in vivo efficacy of mRNA-LNPs. Of the branched lipids, CL4F 8-6 LNPs carrying Cas9 mRNA and sgRNA could achieve 54% genome editing and 77% protein reduction with a single dose of 2.5 mg kg(-1). This mechanism-based data on branched lipids is expected to provide insights into rational lipid design and effective gene therapy in the future.

Quantitative structure-properties relationship analysis of Eigen-value-based indices using COVID-19 drugs structure.

Topological indices are an important method for understanding the fundamental topology of chemical structures. Quantitative structure properties relationship (QSPR) is an analytical approach for breaking down a molecule into a sequence of numerical values that describe the chemical and physical characteristics of the molecule. In this article, we have developed the QSPR analysis between eigenvalue-based topological indices and physical properties of COVID-19 drugs to predict the significance level of eigenvalue based indices. We have to use MATLAB for the computation of indices and SPSS for analysis. We show that positive interia index, signless Laplacian Estrada index and Randic energy are the best predictors of molar reactivity, polar surface area and molecular weight, respectively.

Effects of adding different silver carp fish pastes on the quality of lion's head meatballs

To clarify the most suitable fish paste for preparing lion's head meatballs, this study investigated the effect of adding 6 different silver carp fish pastes: fresh unwashed (group 1-1), fresh washed (group 1-2), frozen unwashed without antifreeze agent (group 2-1), frozen washed without antifreeze agent (group 2-2), frozen unwashed with antifreeze agent (group 3-1), and frozen washed with antifreeze agent (group 3-2) on the basic nutrients, color, texture properties, waterholding capacity, sensory and flavor properties, thiobarbituric acid reactive substance (TBARs) value, and total volatile basic nitrogen (TVB-N) content of pork/fish composite meatballs. The results showed that the contents of protein and fat in meatballs from groups 2-1 and 2-2 were lower than those in groups 3-1 and 3-2, and the contents of water, protein and fat were 61.68%, 11.32% and 19.41% for group 2-1, and 62.45%, 11.09% and 19.33% for group 2-2, respectively. The gel properties, hardness, elasticity, cohesion, and sensory quality of groups 3-1 and 3-2 were significantly higher than those of groups 2-1 and 2-2 (P < 0.05), but there was no significant difference compared with groups 1-1 and 1-2. The odor response value of groups 3 was lower than that of groups 1, and groups 3 had the highest sweetness value (3 039.66) and lowest bitterness value (534.59). The TBARs value and TVB-N content in groups 1-2, 2-2, and 3-2 (with washed fish paste) were significantly lower than those in groups 1-1, 2-1 and 3-1 (with unwashed fish paste) (P < 0.05). Since fresh fish paste is not easy to store and subject to spoilage, frozen washed fish paste with antifreeze agent can be used to produce composite meatballs.

Effect of plastic pollution in soil properties and growth of grass species in semi-arid regions: a laboratory experiment.

Since the year 2020, the use of plastic as a strategy to mitigate the spread of COVID-19 disease has been given substantial attention. Global environmental contamination of plastic creates waste and is a known threat to soil ecosystems as a main sink of microplastics. However, there is still considerable uncertainty about microplastics controlling soil properties alteration. Therefore, we carried out an incubation experiment with soil and Carex stenophylla Wahlenb., which are the dominant soil and grass species in semi-arid regions. We investigated the effect of polymer polyethylene terephthalate (PET) concentrations (0%, 1%, 3%, and 5%) on C. stenophylla growth and soil ammonium-N and nitrate-N, organic matter content, pH, soil aggregates, and soil respiration. When soils were exposed to PET microplastics, fewer seeds germinated (62.8 ± 32%) but not significantly (p value > 0.05) when soils were treated to 0, 1, 3, and 0.5% PET. Shoot height was also not effectively reduced with PET. The soil pH was considerably lower when exposed to higher PET compared to all other treatments with the soil exposed to 5% w/w PET for both unplanted and planted, being 0.84 and 0.54 units, respectively, lower than the controls. The soil microbial respiration under exposure to PET was considerably increased in comparison to control samples. Moreover, the presence of PET resulted in potential alterations of soil stability, and with PET present soil stability increased. In conclusion, PET microplastics cannot significantly affect the development of C. stenophylla but could affect crucial soil properties. In addition, changes occurred with increased variability in soil ammonium-N and nitrate-N, particularly at a high PET ratio.

Growth of a fluid-infused patch from droplet drainage into a thin porous layer

Growth of a fluid-infused patch on a thin porous layer, e.g. on a piece of paper or cloth, is related to the transmission of virus particles through exhaled droplets and aerosols. We present a theoretical model to describe how a wet patch develops gradually through imbibition, once a sessile droplet attaches at a permeable surface and drains gradually into a thin porous layer. Two limiting cases are considered based on different assumptions on the motion of the contact line during the coupled process of drop drainage and patch growth: (i) the apparent contact angle remains unchanged, so the radius of a sessile droplet decreases with time;and (ii) the location of the contact line remains pinned, so the contact angle decreases as time progresses. The model leads to evolution pathways for both the droplet and the fluid film within the porous layer, without introducing arbitrary fitting parameters. Potential implications of the model and its solutions are also discussed briefly in the context of the outspread of COVID-19, employing physical parameters for exhaled droplets, paper and cloth.

Mitigation of microfibers release from disposable masks - An analysis of structural properties.

The use of disposable face masks increased rapidly among the general public to control the COVID-19 spread. Eventually, it increased the disposal of masks and their associated impacts on environmental pollution. Hence, this study aims to analyze the impact of nonwoven fabric structural parameters and weathering on the microfiber release characteristics. Spunbond polypropylene nonwoven with four different weights and meltblown nonwoven with two different weights were used in this study to analyze microfiber release at dry, and wet conditions to simulate improper disposal in the environment. Exposure to sunlight significantly increases the microfiber release from 35 to 50% for spunbond fabric and 56-89% for meltblown fabric. Weathering in sunlight structurally affected the tensile properties of the polypropylene fibers due to photodegradation. The study showed that each mask can produce 1.5 × 102 and 3.45 × 101 mg of microfiber/mask respectively in dry and wet states. In the case of structural parameters, a higher GSM (grams per square meter), abrasion resistance, bursting strength, and thickness showed a positive correlation with microfiber release in both fabrics. Significantly a higher microfiber release was reported with meltblown fabric than the spunbond for a given GSM. The presence of finer fibers and more fibers per unit area in meltblown fabric was noted as the main cause. Nonwoven fabric GSM and the number of fibers in a specific area showed a higher influence on microfiber release. Based on the mask consumption reported in the literature, India alone can produce around 4.27 × 102 tons of microfibers/week as an average of dry and wet conditions. The study suggests that the proper selection of physical parameters can significantly reduce the microfiber fiber release at all stages.

Advanced Particle Removal Technology as an Efficient Tool for Addressing Emerging Global Air Quality and Safety Challenges

Indoor air quality is increasingly recognized as a serious health hazard in many international environments. During the recent pandemic, this concern was amplified as Covid-19-related mortality closely correlated with poor air quality. Even a comparatively small decline in the Air Quality Index (AQI) can be linked to a sharp mortality increase. Worsening air quality levels are compounded by distinct air-quality issues in different geographical areas. In the face of this serious and wide-ranging threat, the common solution-introducing a high MERV-rated filter-comes up short, as these filters create back pressure that often exceeds the capacity of the HVAC systems in which they are installed. High-backpressure filters also use more energy and require frequent filter changes, making them more expensive to maintain and bad for the environment. This paper describes a new form of electrostatic filtration that is ideal for international markets since it has a uniform performance, low back pressure, is energy efficient, and can be tuned to perform at a range of filtration levels depending on demand. Developed at the University of Washington, the technology features porous electrodes that collect and hold, a large capacity of particles regardless of their size and physical properties. This paper will describe the technology, prototype testing, including a 6-month pilot installation, and will detail how the technology can be used to achieve on-demand MERV 15 filtration levels in systems that require continuous low back pressure and reduced energy consumption.

Evaluations of service robots referring to Joruri puppets, and a remote evaluations system

One of the important requirements for service robots is attracting people by attractiveness and to be able to exchange messages with people. With reference to the traditional Japanese puppet show, Ningyo Joruri, we have independently developed OSONO, which is a physical robot, with high-quality choreography. In this paper, we report questionnaire evaluations on OSONO targeting a puppeteer expert group and compare with existing questionnaire results targeting the ordinary person. This shows that the method of creating OSONO and its choreography is effective in widely general. Additionally, we develop a remote evaluations system so that we can conduct questionnaires of OSONO in a short time for more evaluators. We also verify the effectiveness of this remote evaluation system. This system can be expected as a substitute for the conventional face-to-face evaluation, which is becoming difficult to conduct it due to the pandemic of COVID-19. © 2021 IEEE.

Monitoring COVID‐19 through SARS‐CoV‐2 quantification in wastewater: progress, challenges and prospects

Wastewater‐Based Epidemiology (WBE) is widely used to monitor the progression of the current SARS‐CoV‐2 pandemic at local levels. In this review, we address the different approaches to the steps needed for this surveillance: sampling wastewaters (WWs), concentrating the virus from the samples and quantifying them by qPCR, focusing on the main limitations of the methodologies used. Factors that can influence SARS‐CoV‐2 monitoring in WWs include: (i) physical parameters as temperature that can hamper the detection in warm seasons and tropical regions, (ii) sampling methodologies and timetables, being composite samples and Moore swabs the less variable and more sensitive approaches, (iii) virus concentration methodologies that need to be feasible and practicable in simpler laboratories and (iv) detection methodologies that should tend to use faster and cost‐effective procedures. The efficiency of WW treatments and the use of WWs for SARS‐CoV‐2 variants detection are also addressed. Furthermore, we discuss the need for the development of common standardized protocols, although these must be versatile enough to comprise variations among target communities. WBE screening of risk populations will allow for the prediction of future outbreaks, thus alerting authorities to implement early action measurements.

Taking crime guns seriously: A socio-material perspective

This article argues that guns, as objects used in and for crime, have received insufficient criminological attention. It proposes a socio-material perspective for taking crime guns seriously as material agents in the ways many serious crimes are planned and executed. Drawing in part upon affordance theory, the perspective links the ‘objective’ physical properties of guns to their allure and take up for the purposes of carrying out crime. Guns are powerful organising objects in the commission of crime, it is argued, capable of provoking as well as enabling a range of threatening and harmful activities. The perspective is developed drawing upon interview data from a large qualitative study of convicted gun criminals. These data enable the notion of materiality to be considered at different stages of criminal career, particularly prior to first criminal gun use through to enforced or voluntary desistance. The article concludes with a consideration of policy options suggested by the socio-material perspective. In a post-Covid 19 world in which guns have gained greater salience in many countries, it is argued that the need to ‘dematerialise’ gun attraction and use has never been greater.

Influence of Thermal Modification and Impregnation with Biocides on Physical Properties of Italian Stone Pine Wood (Pinus pinea L.)

Featured ApplicationIn recent decades, the importance of wood has also been increasing for outdoor intended use. A more sustainable approach has turned the attention from faraway tropical forests to local wood, that is, European species. Unfortunately, most of the species are not durable;thus, in order to utilize them in outdoor conditions, protection must be provided. Stone pine (Pinus pinea) is one of the most common tree species in the Mediterranean region, with a great potential in applications with higher added value, instead of being used only for burning or packaging. This investigation explores methods for an improvement of the service life of stone pine sapwood using impregnation and modification techniques.The sudden availability of Italian stone pine (Pinus pinea L.) wood due to the infestation of pathogenic fungi and insects highlighted the need to promote its use as a short supply chain resource in Italy and other Mediterranean countries. However, the durability of stone pine sapwood must be enhanced if outdoor use is to be considered. The impregnability of stone pine wood was studied following the standard EN 351-1:2008, using immersion, vacuum, and high-pressure methods with natural waxes and organic solvent-based and copper-amino-based preservative solutions. The efficacy of the impregnation was determined by calculating the kilos of preservative absorbed per cubic meter of wood and by measuring the penetration depth of the preservative. Thermal modifications were carried out using five different maximum temperatures, and their efficacy was determined by measuring the mass loss and color change. Subsequent to thermal treatments, the wood was exposed to a water-vapor-saturated atmosphere, and the increase in mass was calculated for different periods. Stone pine and Scots pine sapwoods showed similar response to the treatments with the exception of soaking impregnation methods where stone pine showed higher uptake, in particular when Silvanolin was used. Our investigation shows that stone pine can be a suitable short supply chain resource in Italy that, when impregnated, could be tested for outdoor use, exactly as is the case with Scots pine.

Assessment of vulnerability and site adaptive capacity to the risk of climate change: the case of Tchogha Zanbil World Heritage earthen site in Iran

Purpose>The aim of this research is to evaluate the vulnerability of earthen heritage when facing climate change, by focusing on Tchogha Zanbil site as a case study – an outstanding example of an earthen site that recurrently faces extreme climatic events. Moreover, the adaptive capacity of traditional knowledge and ancient systems is also evaluated, in order to contribute for future climate change adaptation planning.Design/methodology/approach>The vulnerability of cultural heritage to climate change was considered as the degree to which an identified cultural heritage value was susceptible to, or would be adversely affected by, the effects of climate change, including climate variability and extreme temperatures. In order to establish a vulnerability assessment, this paper will assess different definitions regarding vulnerability, exposure and sensitivity, crossing it with indicators of physical parameters, in order to propose an adaptive capacity for the site, based on ancient traditional knowledge.Findings>Nonetheless, the entailed research helped establish a framework that contributes to outline the vulnerability and the potential for adaptive capacity of World Heritage properties, especially earthen sites located in regions exposed to rising climate change impact.Research limitations/implications>The research faced some limitations regarding access to data and to site visits, due to COVID-19 restrictions that were in place.Originality/value>This research presents a methodological assessment of climate change risk in Tchogha Zanbil, a World Heritage earthen site in Iran, representative of a property highly exposed to risk and vulnerability.

Local Discrimination Based on Piezoelectric Sensing in Robots Composed of Soft Matter with Different Physical Properties

The coronavirus epidemic has attracted significant attention to the applications of pet robots which can be used to treat and entertain people in their homes. However, pet robots are fabricated using hard materials and it is difficult for them to communicate with people through contact. Soft robots are expected to realize communication through contact similar to that of actual pets. Soft robots provide people with a sense of healing and security owing to their softness and can extract rich information through external stimuli by applying a machine learning framework called physical-reservoir computing. It is crucial to determine the differences between the physical properties of soft materials that affect the information extracted from a soft body to develop an intelligent soft robot. In this study, two owl-shaped soft robots with different softnesses were developed to analyze the characteristics of the signal data obtained via piezoelectric film sensors embedded in models with different physical properties. An accuracy of 94.2% and 95.9% was obtained for touched part classification using 1D CNN and logistic regression models, respectively. Additionally, the relationship between the softness of material and classification performance was investigated by comparing the distribution of part classification accuracy for different hyper-parameters of two owl models.

Understanding and Targeting Breast Cancer Metastasis Initiating Circulating Tumor Cells and Niches

One of the biggest challenges in breast cancer is how to prevent and treat metastasis. Ourmain goal is to identify cell intrinsic and extrinsic metastasis-promoting mechanisms anddevelop novel therapeutics for targeting metastasis. We aims to investigate the molecular andphysical properties of metastasis-initiating circulating tumor cells (CTCs), the uniqueproperties of metastasis-supporting niches, and the mechanism of immune evasion in CTCs. Thispast year is the first year of this funding award and the COVID-19 pandemic significantlyimpacted our research activities in a negative way. Despite the challenges and unusualcircumstances, we still made progress in analyzing CTC heterogeneity in morphology andtranscriptomes at the single cell level in our existing patient-derived CTC lines,optimization of the spatial transcriptomic profiling protocols for PDX tissues, as well asinvestigation of the mechanisms of hypoxia-mediated inhibition of CTC intrinsic interferonand antigen presentation signals.

Morphological, chemical, and physical characteristics of the ethiopian highland (yushania alpina) bamboo

This research paper aims to characterize and evaluate the morphological as well as the physical proper-ties of Ethiopian Y. Alpina bamboo based on the effect of age and height. The bamboo has been harvested in the Amhara region in the Injibara town in January 2020. Nine bamboo samples have collected at the age of 1, 2, and 3 years old. Bamboo Culm has been divided into three positions along with Culm height like bottom, middle, and top. Analysis of the significance of age and height on physical properties has been done using Stata.12 software with linear regression and ANCOVA. Both morphology and physical properties have been used to improve understanding of bamboo's performance used as a structural appli-cation, bamboo composite processing, fiber extraction process, bamboo laminated processing, and furni-ture making materials. Age of 1-year-old bamboo has high Culm diameter, wall thickness, Internode length compared to the age of 2 and 3 years old bamboo. When bamboo generates new shoots under the rhizome, new shoots generate and grow high morphological properties of bamboo compared to the previous old bamboo. Percentage of moisture content, shrinkage in wall thickness, and shrinkage in Culm diameter decrease when the age of bamboo increases. Furthermore, these properties have been decreased along with Culm height from bottom to the top position. Oven-dry density increase with the age of bamboo increase, whereas its property has been decreased along with Culm height from bottom to the top position. Cellulose content increase when the ages of Culm older, however, extractive and ash content decrease when the ages of Culm older. From the greatest to lowest of hemicelluloses, and lignin contents are at the ages of 2, 3, and 1 year-old respectively. (c) 2021 Elsevier Ltd. All rights reserved. Selection and peer-review under responsibility of the scientific committee of the 5th International Con-ference on Recent Advances in Material Chemistry.

Superheated Steam Torrefaction of Biomass Residues with Valorisation of Platform Chemicals—Part 1: Ecological Assessment

Within the last decade, research on torrefaction has gained increasing attention due to its ability to improve the physical properties and chemical composition of biomass residues for further energetic utilisation. While most of the research works focused on improving the energy density of the solid fraction to offer an ecological alternative to coal for energy applications, little attention was paid to the valorisation of the condensable gases as platform chemicals and its ecological relevance when compared to conventional production processes. Therefore, the present study focuses on the ecological evaluation of an innovative biorefinery concept that includes superheated steam drying and the torrefaction of biomass residues at ambient pressure, the recovery of volatiles and the valorisation/separation of several valuable platform chemicals. For a reference case and an alternative system design scenario, the ecological footprint was assessed, considering the use of different biomass residues. The results show that the newly developed process can compete with established bio-based and conventional production processes for furfural, 5-HMF and acetic acid in terms of the assessed environmental performance indicators. The requirements for further research on the synthesis of other promising platform chemicals and the necessary economic evaluation of the process were elaborated.