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1.
Chemical Science ; : 16, 2022.
Article in English | Web of Science | ID: covidwho-1886244

ABSTRACT

Monoclonal antibodies are emerging as a viable treatment for the coronavirus disease 19 (COVID-19). However, newly evolved variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can reduce the efficacy of currently available antibodies and can diminish vaccine-induced immunity. Here, we demonstrate that the microscopic dynamics of neutralizing monoclonal antibodies can be profoundly modified by the mutations present in the spike proteins of the SARS-COV-2 variants currently circulating in the world population. The dynamical perturbations within the antibody structure, which alter the thermodynamics of antigen recognition, are diverse and can depend both on the nature of the antibody and on the spatial location of the spike mutation. The correlation between the motion of the antibody and that of the spike receptor binding domain (RBD) can also be changed, modulating binding affinity. Using protein-graph-connectivity networks, we delineated the mutant-induced modifications in the information-flow along allosteric pathway throughout the antibody. Changes in the collective dynamics were spatially distributed both locally and across long-range distances within the antibody. On the receptor side, we identified an anchor-like structural element that prevents the detachment of the antibodies;individual mutations there can significantly affect the antibody binding propensity. Our study provides insight into how virus neutralization by monoclonal antibodies can be impacted by local mutations in the epitope via a change in dynamics. This realization adds a new layer of sophistication to the efforts for rational design of monoclonal antibodies against new variants of SARS-CoV2, taking the allostery in the antibody into consideration.

2.
Aloma-Revista De Psicologia Ciencies De L Educacio I De L Esport ; 40(1):23-34, 2022.
Article in Spanish | Web of Science Web of Science | ID: covidwho-1885013

ABSTRACT

In mid-March 2020, the world suddenly stopped with the appearance of COVID-19. However, the need to continue formal education, go to work and maintain communication with others remained intact. Technologies have made it possible to carry out these activities digitally in a space of interaction called cyber-coexistence. This research analyses the psychometric properties of a questionnaire intended to measure cyber-coexistence in the context of teaching and learning processes during the health crisis. Recommendations based on the literature were considered in the construction and validation of the instrument, which was carried out through expert judgement and exploratory and confirmatory factor analysis. The instrument was administrated in June 2020 remotely to a convenience sample of 400 secondary and tertiary students from schools in the Biobio region, Chile. Factor analyses yielded a latent structure with an adequate fit to the data, consisting of 23 items divided into six underlying dimensions of the construct. Reliability analysis yields an internal consistency of .76 according to the Omega coefficient. Orientations for the regulation of the use of mobile devices to promote positive cyber-coexistence in educational context are offered.

3.
Journal of Information Technology Education-Research ; 21:217-244, 2022.
Article in English | English Web of Science | ID: covidwho-1884933

ABSTRACT

Aim/Purpose The goal of the study was to investigate whether the COVID-19 pandemic and its effects on the educational process also affected the views, attitudes, and intentions of educators regarding the use of computers and their applications both for professional and personal purposes. Background A model was developed and tested that included the factors present in the Technology Acceptance Model, together with self-efficacy, and the participant's gender, age group, level of studies, and ICT training. The educators' views were recorded twice: (i) before the lockdowns and (ii) after the lockdowns. The two resulting models were compared, so as to come to conclusions regarding possible changes. Methodology The target group was Greek educators (i.e., individuals teaching in primary and secondary education). The study was conducted in two stages: (i) at the beginning of the first general lockdown (March 2020), and (ii) at the beginning of the 2021 school year (September 2021), when schools re-opened and resumed their normal operations. The final sample was 1,099 educators. A questionnaire was used having three sections: (i) four demographic questions, (ii) twenty-five items for recording the educators' views, attitudes, and intentions, and (iii) two questions for recording how many hours per -typical day they use computers for professional and personal purposes. Contribution The study contributes to the deeper understanding of educators' views, attitudes, intentions, and actual use of computers and how and to what extent these changed after the period of the multiple lockdowns due to the COVID-19 pandemic. Findings The study's major findings were: The lockdowns did not significantly affect the educators' views, attitudes, and intention to use computers, but resulted in a more extensive use of computers for work than before. The results oppose the basic assumption of TAM that the behavioral intention to use a technological tool translates into the actual use of this tool. The perceived usefulness of computers shaped the educators' behavioral intention to use them. Computer self-efficacy shaped the attitude of educators toward computers. After the pandemic, the educators' age and gender no longer play a role in shaping their views. The additional ICT training the educators received did not have an effect on any factor. Recommendations for Practitioners Governments have to equip schools with computers and provide the framework that fosters their use. Strategies and support mechanisms that make educators more confident in the use of ICTs and also provide evidence for their usefulness should be implemented. The above will allow the educators' behavioral intention to use ICTs to be translated into actual use. Administrators in higher education should consider the inclusion of more ICT-related courses in their undergraduate and postgraduate programs addressed to educators. Changes have to be made to the context and content of the in-service ICT training programs. Recommendations for Researchers The inclusion of self-efficacy is recommended in future studies involving TAM. As deviations from the original TAM were noted, possible swifts in how educators' views and intentions are shaped need to be further investigated. Impact on Society The increased use of computers by educators might lead to a more intense use of ICTs in schools, resulting in an education better aligned with the needs of the digitalized society. Future Research Comparative studies, targeting educators from other countries, will help to understand how the pandemic affected the educational systems worldwide. Studies are needed for verifying the model's validity and applicability. Researchers can consider the inclusion of other factors that might have a significant effect. Qualitative data may offer an in-depth understanding of educators' beliefs. Finally, longitudinal studies can help to understand whether the pandemic had a lasting effect on educators' intention to use computers.

4.
Vet Sci ; 9(6)2022 Jun 05.
Article in English | MEDLINE | ID: covidwho-1884450

ABSTRACT

SARS-CoV-2 emerged in 2019 and found diagnostic laboratories unprepared worldwide. To meet the need for timely and accurate virus detection, laboratories used rapid Ag tests and PCR kits based on costly multi-channel real-time techniques. This study aimed to develop a conventional nested PCR based on the SARS-CoV-2 N gene, validate it against some approved assays, and apply it to samples from six cats with respiratory symptoms obtained in early 2020 during the first COVID-19 wave in humans in Bulgaria. The nested PCR technique showed 100% sensitivity and specificity; it could detect extracted SARS-CoV-2 RNA at concentrations as low as 0.015 ng/µL. The results identified the six tested cat samples as positive. Sequence analysis performed in two of them confirmed this. The presented technique is reliable, easy to implement and inexpensive, and can be successful in strategies for the prevention and control of SARS-CoV-2 in humans, cats and other susceptible species.

5.
Molecules ; 27(11)2022 Jun 02.
Article in English | MEDLINE | ID: covidwho-1884284

ABSTRACT

Salicylic acid is a key compound in nonsteroidal anti-inflammatory drugs that has been recently used for preventing the risk of hospitalization and death among COVID-19 patients and in preventing colorectal cancer (CRC) by suppressing two key proteins. Understanding drug-drug interaction pathways prevent the occurrence of adverse drug reactions in clinical trials. Drug-drug interactions can result in the variation of the pharmacodynamics and pharmacokinetic of the drug. Inhibition of the Cytochrome P450 enzyme activity leads to the withdrawal of the drug from the market. The aim of this paper was to develop and validate an HPLC-UV method for the quantification of 4'-hydroxydiclofenac as a CYP2C9 metabolite using salicylic acid as an inhibitor in rat liver microsomes. A CYP2C9 assay was developed and validated on the reversed phase C18 column (SUPELCO 25 cm × 4.6 mm × 5 µm) using a low-pressure gradient elution programming at T = 30 °C, a wavelength of 282 nm, and a flow rate of 1 mL/min. 4'-hydroxydiclofenac demonstrated a good linearity (R2 > 0.99), good reproducibility, low detection, and quantitation limit, and the inter and intra-day precision met the ICH guidelines (<15%). 4'-hydroxydiclofenac was stable for three days and showed an acceptable accuracy and recovery (80-120%) within the ICH guidelines in a rat liver microsome sample. This method will be beneficial for future applications of the in vitro inhibitory effect of salicylic acid on the CYP2C9 enzyme activity in rat microsomes and the in vivo administration of salicylic acid in clinical trials.


Subject(s)
Diclofenac , Microsomes, Liver , Animals , Chromatography, High Pressure Liquid/methods , Cytochrome P-450 CYP2C9 , Diclofenac/analogs & derivatives , Diclofenac/analysis , Drug Interactions , Humans , Rats , Reproducibility of Results , Salicylic Acid/pharmacology
6.
Sage Open ; 12(2):16, 2022.
Article in English | English Web of Science | ID: covidwho-1883497

ABSTRACT

This study examined Vietnamese local residents' attitudes toward the reopening of their country to international tourists amid COVID-19 from March to April 2021. It began with a qualitative analysis of local residents' opinions (n = 240) to identify the factors that could affect their attitudes, then continued with a review of the literature to create a theoretical model. Finally, the study implemented a structured survey to collect quantitative data (n = 412) to confirm the model. The outcomes revealed that "perceived vaccine efficacy" and "xenophobia" were two significant predictors of "attitude toward inbound tourism." Implications of this study were then discussed.

7.
Minerva Orthopedics ; 73(2):225-229, 2022.
Article in English | Scopus | ID: covidwho-1879754

ABSTRACT

a B s t r a c t BacKGrOUNd: due to the cOVid-19 pandemic, the global higher-education community has been pushed into an unplanned experiment in online learning and the large number of hours spent sitting studying led students to develop back pain, which can result in an altered perception of the body. the Fremantle Back awareness Questionnaire (FreBaQ) is tool to assess back-specific altered body perception. The primary objective of the study was to evaluate validity and reliability of the FreBaQ in the assessment of the perception of the spinal column following the change in the method of carrying out the teaching activity of the degree courses during the lockdown due to the cOVid-19 pandemic. METHODS: Internal consistency was evaluated with Cronbach’s α coefficient, while for criterion validity, the Pearson correlation coefficient of the FreBAQ was evaluated with the SF-12 and the Perceived Stress Scale (PSS). resULts: the FreBaQ was applied to 524 individuals with a mean age of 23 years (sd 4.8). the internal consistency showed good values, with an alpha coefficient of 0.87 as regards the total population, and an alpha coefficient of 0.77 as regards individuals with spinal diseases. the validity study demonstrated good linear correlation with the sF-12 (p<0.01) and with the perceived stress scale (p<0.01). CONCLUSIONS: The present findings revealed the FreBAQ as a reliable and valid tool to assess back-specific altered body perception. © 2021 ediZiONi MiNerVa Medica

8.
Journal of Hospitality and Tourism Technology ; 13(3):559-572, 2022.
Article in English | ProQuest Central | ID: covidwho-1878911

ABSTRACT

Purpose>This study aims to incorporate innovative technology (i.e. blockchain) into the design of an online travel agency (B-OTA). The authors accelerated the design of the prototype system through expert interviews to understand the convenience of the blockchain travel and accommodation platform for users. The authors further validated the prototype system design and improved reference capability through usability testing.Design/methodology/approach>Rapid prototyping of B-OTA was carried out through the Delphi method and literature compilation. Design validation of usability testing was conducted using modified systematic evaluation methodology. Prototype system suggestions and improvements were collected through feedback from test subjects.Findings>This study will be helpful for the development of B-OTAs and related technologies. Through usability testing, the authors verified that creating a familiar operating environment for users can reduce users’ fear of unfamiliar technology. The results of this research provide future B-OTA researchers and practitioners with design guidelines.Originality/value>Beyond the blockchain-related technologies used, few studies have reported experiences and interface design of B-OTAs. This study states that, as many human–computer interaction-related papers have pointed out, the basic prerequisite for successful interface design is a clearer definition of users’ needs during operation. User experience and user interface design should provide a good user experience.

9.
J Patient Rep Outcomes ; 6(1): 41, 2022 May 04.
Article in English | MEDLINE | ID: covidwho-1879267

ABSTRACT

BACKGROUND: At the onset of the COVID-19 pandemic, there was limited understanding of symptom experience and disease progression. We developed and validated a fit-for-purpose disease-specific instrument to assess symptoms in patients with COVID-19 to inform endpoints in an interventional trial for non-hospitalized patients. METHODS: The initial drafting of the 23-item Symptoms Evolution of COVID-19 (SE-C19) Instrument was developed based on the Centers for Disease Control and Prevention symptom list and available published literature specific to patients with COVID-19 as of Spring 2020. The measurement principles outlined in the Food and Drug Administration (FDA) Patient-Reported Outcomes (PRO) guidance and the FDA's series of four methodological Patient-Focused Drug Development guidance documents were also considered. Following initial development, semi-structured qualitative interviews were conducted with a purposive sample of 30 non-hospitalized COVID-19 patients. Interviews involved two stages: (1) concept elicitation, to obtain information about the symptoms experienced as a result of COVID-19 in the patients' own words, and (2) cognitive debriefing, for patients to describe their understanding of the SE-C19 instructions, specific symptoms, response options, and recall period to ensure the content of the SE-C19 is relevant and comprehensive. Five clinicians treating COVID-19 outpatients were also interviewed to obtain their insights on symptoms experienced by patients and provide input on the SE-C19. RESULTS: Patients reported no issues regarding the relevance or appropriateness of the SE-C19 instructions, including the 24-h recall period. The comprehensiveness of the SE-C19 was confirmed against the conceptualization of the patient experience of symptoms developed in the qualitative research. Minor conceptual gaps were revealed to capture nuances in the experience of nasal and gustatory symptoms and systemic manifestations of sickness. Almost all items were endorsed by patients as being appropriate, well understood, and easy to respond to. The clinicians largely approved all items, response options, and recall period. CONCLUSIONS: The qualitative research provided supportive evidence of the content validity of the SE-C19 to assess the symptoms of outpatients with COVID-19, and its use in clinical trials to evaluate the benefit of treatment. Minor changes may be considered to improve conceptual clarity and ease of responding.

10.
21st International Conference on Image Analysis and Processing, ICIAP 2022 ; 13231 LNCS:197-209, 2022.
Article in English | Scopus | ID: covidwho-1877765

ABSTRACT

Since the beginning of the COVID-19 pandemic, more than 350 million cases and 5 million deaths have occurred. Since day one, multiple methods have been provided to diagnose patients who have been infected. Alongside the gold standard of laboratory analyses, deep learning algorithms on chest X-rays (CXR) have been developed to support the COVID-19 diagnosis. The literature reports that convolutional neural networks (CNNs) have obtained excellent results on image datasets when the tests are performed in cross-validation, but such models fail to generalize to unseen data. To overcome this limitation, we exploit the strength of multiple CNNs by building an ensemble of classifiers via an optimized late fusion approach. To demonstrate the system’s robustness, we present different experiments on open source CXR datasets to simulate a real-world scenario, where scans of patients affected by various lung pathologies and coming from external datasets are tested. Promising performances are obtained both in cross-validation and in external validation, obtaining an average accuracy of 93.02% and 91.02%, respectively. © 2022, The Author(s), under exclusive license to Springer Nature Switzerland AG.

11.
International Journal of Emerging Technology and Advanced Engineering ; 12(5):88-95, 2022.
Article in English | Scopus | ID: covidwho-1876623

ABSTRACT

The process of Teaching and Learning in times of Covid-19 brought experiences between teachers and students, seeking to improve with some teaching method to obtain satisfaction in their learning, the objective of this research is to validate the instrument of measurement of Teaching and Learning Experience Questionnaire (ETLQ) in the Peruvian context with university students, This instrument consists of three parts, the Teaching and Learning Environment which consists of 19 questions, the Student Learning Approach which consists of 9 questions and the Critical Thinking with 4 questions, each question is composed of 5-point Likert-type scales (1 point = "Strongly disagree" and 5 points = "Strongly agree". A total of 2027 university students participated throughout Peru, including students from national and private universities. Cronbach's Alpha =0.957 with 32 numbers of items was valid for its respective use, with which it will be possible to measure the Learning Experiences in university students, reinforcing this result with different tests whose results were optimal and reliability analysis. Concluding that, the validation instrument is valid in the Peruvian environment, online education is accepted in its highest percentage and that the rejection of this is only in the most vulnerable students and the feedback to students is the most outstanding. © 2022 IJETAE Publication House. All Rights Reserved.

12.
Indian J Med Res ; 2022 May 20.
Article in English | MEDLINE | ID: covidwho-1875899

ABSTRACT

Background & objectives: COVID-19 pandemic has triggered social stigma towards individuals affected and their families. This study describes the process undertaken for the development and validation of scales to assess stigmatizing attitudes and experiences among COVID-19 and non-COVID-19 participants from the community. Methods: COVID-19 Stigma Scale and Community COVID-19 Stigma Scale constituting 13 and six items, respectively, were developed based on review of literature and news reports, expert committee evaluation and participants' interviews through telephone for a multicentric study in India. For content validity, 61 (30 COVID-19-recovered and 31 non-COVID-19 participants from the community) were recruited. Test-retest reliability of the scales was assessed among 99 participants (41 COVID-19 recovered and 58 non-COVID-19). Participants were administered the scale at two-time points after a gap of 7-12 days. Cronbach's alpha, overall percentage agreement and kappa statistics were used to assess internal consistency and test-retest reliability. Results: Items in the scales were relevant and comprehensible. Both the scales had Cronbach's α above 0.6 indicating moderate-to-good internal consistency. Test-retest reliability assessed using kappa statistics indicated that for the COVID-19 Stigma Scale, seven items had a moderate agreement (0.4-0.6). For the Community COVID-19 Stigma Scale, four items had a moderate agreement. Interpretation & conclusions: Validity and reliability of the two stigma scales indicated that the scales were comprehensible and had moderate internal consistency. These scales could be used to assess COVID-19 stigma and help in the development of appropriate stigma reduction interventions for COVID-19 infected, and mitigation of stigmatizing attitudes in the community.

13.
Healthcare (Basel) ; 10(5)2022 Apr 21.
Article in English | MEDLINE | ID: covidwho-1875535

ABSTRACT

Patient satisfaction after total joint arthroplasties (TJA) represents a key element for the evaluation of surgery success in relation to subjects' needs and expectations. The assessment tools are applied inconsistently throughout the literature, and thus, it is difficult to compare results among different studies. Goodman et al. proposed a standardized questionnaire with strong psychometric properties for the assessment of satisfaction. The present study aims to translate, adapt, and validate the Goodman questionnaire for the Italian population. After translation and back translation, the questionnaire was administrated to 50 patients. Internal consistency, test-retest reliability, floor and ceiling effects, and construct validity were evaluated (correlation with KOOS/HOOS, SF-12 PCS/MCS, EQ-5D). Responsiveness was evaluated with respect to SF-12 PCS improvements. The Italian version of the Goodman score questionnaire demonstrated psychometric properties similar to those of the original version. The translated questionnaire showed good internal consistency (Cronbach's alpha = 0.836) and test-retest reliability (ICC: 0.507). Moderate/strong correlations were observed between the Italian version of the Goodman score and other scores. The score significantly discriminated patients who improved from those who did not improve in SF-12 PCS after treatment. This study provides an adapted and validated Italian version of the Goodman score questionnaire, with psychometric properties similar to those of its original counterpart.

14.
16th Annual IEEE International Systems Conference, SysCon 2022 ; 2022.
Article in English | Scopus | ID: covidwho-1874344

ABSTRACT

Functional integration of human cognition and machine reasoning is an industry-wide problem where failure risks health or safety. Differences in human versus machine functioning obscure conventional integration. We introduce cognitive work problems (CWP) for rigorous, verifiable functional integration. CWP specify the cognitive problem that integrated designs must solve. They are technology-neutral, work objects, allowing people and computing to share and transform them in coordination. The end-to-end method is illustrated on a system that employs AI for remote patient monitoring (RPM) during COVID-19 home care. The CWP specified actionable risk awareness as the medical problem RPM must solve. Graphical modeling standards enabled user participation: CWP as finite state machines and system behavior in BPMN. For model checking, the CWPs logical content was translated to linear temporal logic (LTL) and the BPMN into Promela as inputs to the SPIN model checker. SPIN verified the Promela implements the LTL correctly. We conclude this CWP-derived RPM design solves the medical problem and enhances patient safety. The method appears general to many critical systems. © 2022 IEEE.

15.
Infection ; 2021 Nov 19.
Article in English | MEDLINE | ID: covidwho-1872762

ABSTRACT

PURPOSE: To externally validate four previously developed severity scores (i.e., CALL, CHOSEN, HA2T2 and ANDC) in patients with COVID-19 hospitalised in a tertiary care centre in Switzerland. METHODS: This observational analysis included adult patients with a real-time reverse-transcription polymerase chain reaction or rapid-antigen test confirmed severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection hospitalised consecutively at the Cantonal Hospital Aarau from February to December 2020. The primary endpoint was all-cause in-hospital mortality. The secondary endpoint was disease progression, defined as needing invasive ventilation, ICU admission or death. RESULTS: From 399 patients (mean age 66.6 years ± 13.4 SD, 68% males), we had complete data for calculating the CALL, CHOSEN, HA2T2 and ANDC scores in 297, 380, 151 and 124 cases, respectively. Odds ratios for all four scores showed significant associations with mortality. The discriminative power of the HA2T2 score was higher compared to CALL, CHOSEN and ANDC scores [area under the curve (AUC) 0.78 vs. 0.65, 0.69 and 0.66, respectively]. Negative predictive values (NPV) for mortality were high, particularly for the CALL score (≥ 6 points: 100%, ≥ 9 points: 95%). For disease progression, discriminative power was lower, with the CHOSEN score showing the best performance (AUC 0.66). CONCLUSION: In this external validation study, the four analysed scores had a lower performance compared to the original cohorts regarding prediction of mortality and disease progression. However, all scores were significantly associated with mortality and the NPV of the CALL and CHOSEN scores in particular allowed reliable identification of patients at low risk, making them suitable for outpatient management.

16.
Vaccines ; 10(5):682, 2022.
Article in English | ProQuest Central | ID: covidwho-1871300

ABSTRACT

Vaccine literacy (VL) mediates the transfer of information and facilitates vaccination acceptance. The aims of this study are to validate the HLVa-IT (Health Literacy Vaccinale degli adulti in Italiano—Vaccine health literacy for adults in Italian language) for the staff of nursing homes (NHs), to measure VL in such a peculiar target group, and to assess its relationship with the sources used to obtain information about vaccines and vaccinations. A survey has been conducted in a sample of Tuscan NHs using an online questionnaire. Eight-hundred and fifty-three questionnaires were analyzed. Two dimensions of the HLVa-IT appeared (functional and interactive/communicative/critical VL). The HLVa-IT interactive/communicative/critical subscale score was slightly higher than the functional subscale, although with no statistical significance. General practitioners (GPs) or other professionals have been reported as the main source of information by most of the respondents (66.1%). The HLVa-IT total score was significantly higher among those who have declared to use official vaccination campaigns (mean score: 3.25 ± 0.49;p < 0.001), GPs or other health professionals (3.26 ± 0.47;p < 0.001), and search engines (3.27 ± 0.48;p = 0.040) as the main sources of information. In conclusion, the HLVa-IT could be reliable test to investigate VL for staff of NHs, and also to highlight criticalities related to information sources.

17.
Sustainability ; 14(10):5940, 2022.
Article in English | ProQuest Central | ID: covidwho-1871126

ABSTRACT

This research conducted three studies to develop a scale for measuring the service quality of fresh food delivery platforms. In Study 1, the scale development stage, a total of 55 items were generated via literature reviews, text mining, and expert interviews. In Study 2, the preliminary assessment stage, the first consumer survey (n = 550) was conducted to purify and refine the items derived from Study 1 using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). Lastly, in Study 3, the second consumer survey (n = 570) was carried out to validate the scale using CFA. Ultimately, Food PlatQual scale, consisting of 25 items with seven dimensions: “information quality”, “price”, “product assortment”, “problem resolution”, “delivery quality”, “ease of use”, and “trendiness”. The current study is expected to offer a theoretical basis for future research as well as offer useful managerial implications for sustainable fresh food delivery platform services.

18.
Mediterranean Journal of Clinical Psychology ; 10(1):20, 2022.
Article in English | Web of Science | ID: covidwho-1870126

ABSTRACT

Background: COVID-19 pandemic placed unusual additional burden upon international healthcare systems. This study aims to explore the associations between burnout, alexithymia and defense mechanisms in a group of Italian hospital physicians during the COVID-19 pandemic. Methods: 96 hospital physicians completed self-reported questionnaires through Google Forms platform, including Maslach Burnout Inventory (MBI), Defense Style Questionnaire-40 (DSQ-40), and 20- item Toronto Alexithymia Scale (TAS-20). Results: Emotional Exhaustion (EE) and Depersonalization (DP) burnout dimensions were positively correlated with alexithymia and with immature and neurotic defenses, while negative associations were correlated with a mature defensive style. MBI Personal Accomplishment (PA) was negatively correlated with alexithymia levels but positively correlated with mature defenses. According to regression models, EE levels were predicted by female gender (beta = -0.20;p <.04) and DSQ mature defenses (beta= -.24;p<.02);DP levels were predicted by alexithymia total score (beta= 0.26;p <.04) and DSQ mature defenses (beta= -.20;p<.05);and PA levels were predicted by alexithymia total score (beta = -0.29;p <.02) and DSQ mature defense (beta=.45;p<.001). Conclusions: Consistent with the broader literature, an association between burnout and both alexithymia and defense mechanisms emerged. These findings highlight the importance of reducing occupational-related burden on healthcare workers and of promoting protecting strategies to deal with emergency situations.

19.
Journal of Oncology Pharmacy Practice ; 28(2 SUPPL):32-33, 2022.
Article in English | EMBASE | ID: covidwho-1868961

ABSTRACT

Background: A review of SACT services across SCAN was undertaken from 2016-2108 with the aim of identifying sustainable service models to future proof personcentred, safe and effective care across the network.1 A key objective from the review was to identify a suitable tool which would measure resource capacity within day units and aid future planning. A review of literature identified no off-the-shelf tool therefore a regional group was set up to develop one, building on work completed in West Of Scotland Cancer Network (2018). All health boards and disciplines were represented. The SCAN SACT Capacity & Future Modelling Tool is the outcome of this work and was launched for use in 2020. Methodology • All SACT regimes delivered in day units / Out-patient departments across SCAN listed. • Inclusion of non-SACT supportive interventions. • Chair, nurse, clinical pharmacist and pharmacy isolator resource applied to each regime based on regional time and motion studies and national guidance. • Non-patient facing activities for service delivery identified for inclusion. • Capacity modifiers agreed and included for staff in training, leave, human factors. • Individual unit parameters applied e.g., opening hours, staffing, chairs. • Activity data drawn from local ChemoCare® system. • Regional and Board level validation. • Input from workforce planning analyst team to maximise functionality and usability. • Presented to Regional Workforce Planning Group and Scottish Oncology Pharmacy Group for external input and validation. • Development of a user manual, standard operating procedures and a business-as-usual process for maintenance and bi-annuals updates. Discussion: A beta testing phase was completed by January 2020 to validate formula, content of manual, activity reports and usability. COVID-19 caused a delay in launch, but the tool was updated to include new regimes and was rolled out in 2021. The tool can be customised for individual cancer units and is endorsed at regional executive level for: • Monitoring the impact of activity on chair, aseptic and clinical pharmacy, and nursing resource. • Aiding individual units to model the impact of practice change, service redesign and new medicines. • Supporting the understanding of capacity across the region. Outputs from the tool have contributed to successful business cases for staff, chair resource and to explore the impact of new regimens for formulary applications. Next steps would be to expand the tool to include more trial regimens and explore potential for links with national work streams through interest generated to date. Conclusion: Clinical and management teams are embracing the tool for service planning, moving from a process which focused mainly on numbers and finance to one which interprets those numbers in terms of environmental and human resource. As the patient population we care for and therapies we deliver become more complex this enhancement to service planning supports both patient and professional safety.2.

20.
Pharmaceuticals (Basel) ; 15(6)2022 May 27.
Article in English | MEDLINE | ID: covidwho-1869735

ABSTRACT

MPI8, a peptidyl aldehyde, is a potent antiviral agent against coronavirus. Due to unique tri-peptide bonds and the formyl functional group, the bioassay of MPI8 in plasma was challenged by a strong interference from water MPI8. Using QTOF LC-MS/MS, we identified MPI8•H2O as the major interference form that co-existed with MPI8 in aqueous and biological media. To avoid the resolution of MPI8 and MPI8•H2O observed on reverse phase columns, we found that a Kinetex hydrophilic interaction liquid chromatography (HILIC) column provided co-elution of both MPI8 and MPI8•H2O with a good single chromatographic peak and column retention of MPI8 which is suitable for quantification. Thus, a sensitive, specific, and reproducible LC-MS/MS method for the quantification of MPI8 in rat plasma was developed and validated using a triple QUAD LC-MS/MS. The chromatographic separation was achieved on a Kinetex HILIC column with a flow rate of 0.4 mL/min under gradient elution. The calibration curves were linear (r2 > 0.99) over MPI8 concentrations from 0.5-500 ng/mL. The accuracy and precision are within acceptable guidance levels. The mean matrix effect and recovery were 139% and 73%, respectively. No significant degradation of MPI8 occurred under the experimental conditions. The method was successfully applied to a pharmacokinetic study of MPI8 after administration of MPI8 sulfonate in rats.

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