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Anaerobe ; 70: 102389, 2021 Aug.
Article in English | MEDLINE | ID: covidwho-1242868


Botulism is a neuroparalytic syndrome caused by a neurotoxin produced by Clostridium botulinum. We describe a patient with neurological symptoms associated with intoxication by Clostridium botulinum and infection by SARSCoV2. This report underlines that it is mandatory, even in case of SARS-CoV-2 positivity, to investigate all the causes of a clinical pattern.

Botulism/diagnosis , COVID-19/epidemiology , Adolescent , Botulism/microbiology , COVID-19/virology , Clostridium botulinum/genetics , Clostridium botulinum/isolation & purification , Diagnosis, Differential , Female , Humans , Pandemics , SARS-CoV-2/physiology
Int J Environ Res Public Health ; 18(9)2021 04 21.
Article in English | MEDLINE | ID: covidwho-1231452


Botulinum neurotoxins are considered as one of the most potent toxins and are produced by Clostridium botulinum. It is crucial to have a rapid and sensitive method to detect the bacterium Clostridium botulinum in food. In this study, a rapid detection assay of C. botulinum in food using loop-mediated isothermal amplification (LAMP) technology was developed. The optimal primers were identified among three sets of primers designed specifically based on the partial ntnh gene encoding nontoxic-nonhaemagglutinin (NTNH) for rapid detection of the target DNA in plasmids. The optimal temperature and reaction time of the LAMP assay were determined to be 64 °C and 60 min, respectively. The chemical kit could be assembled based on these optimized reaction conditions for quick, initial high-throughput screening of C. botulinum in food samples. The established LAMP assay showed high specificity and sensitivity in detecting the target DNA with a limit of 0.0001 pg/ul (i.e., ten times more sensitive than that of the PCR method) and an accuracy rate of 100%. This study demonstrated a potentially rapid, cost-effective, and easy-operating method to detect C. botulinum in food and clinical samples based on LAMP technology.

Botulinum Toxins , Clostridium botulinum/isolation & purification , Food Contamination/analysis , Botulinum Toxins/genetics , Clostridium botulinum/genetics , DNA Primers , Molecular Diagnostic Techniques , Nucleic Acid Amplification Techniques , Sensitivity and Specificity