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BMC Microbiol ; 21(1): 283, 2021 10 18.
Article in English | MEDLINE | ID: covidwho-1477261


BACKGROUND: The widespread use of shared bicycles has increased the demand and sanitary requirements for shared bicycles. Previous studies have identified potentially pathogenic bacteria on the surfaces of shared bicycles, but fungal communities have not been investigated. METHODS: We sampled shared-bicycle handles and saddles from five selected locations in a metropolis (Chengdu, China, n = 98) and used surrounding air deposition samples as controls (n = 12). Full-length ITS sequencing and multiple bioinformatic analyses were utilized to reveal fungal community structures and differences. RESULTS: Aspergillus was dominant on both the handles and saddles of shared bicycles, and Alternaria and Cladosporium were the most abundant families in the air samples. Significant differences in fungal community structures were found among the three groups. The handle samples contained higher abundances of Aureobasidium melanogenum and Filobasidium magnum than the saddle and air samples. The saddle samples had a higher abundance of Cladosporium tenuissimum than the other two sample types (P < 0·05). A higher abundance of fungal animal pathogens on shared-bicycle surfaces than in air by FUNGuild (P < 0·05). Moreover, the co-occurrence network of fungi on handles was more stable than that on saddles. CONCLUSION: There were more potential pathogens, including Aspergillus pseudoglaucus, Aureobasidium melanogenum, Kazachstania pintolopesii, Filobasidium magnum, Candida tropicalis, and Malassezia globose were found on shared bicycles than in air, suggesting that hands should not contact mucous membrane after cycling, especially in susceptible individuals, and hygiene management of shared bicycles should be given more attention by relevant organizations worldwide.

Bicycling , Mycobiome , Air Microbiology , China , Cities , Cluster Analysis , DNA, Fungal/genetics , DNA, Ribosomal Spacer/genetics , Fungi/classification , Fungi/genetics , Fungi/isolation & purification , Fungi/pathogenicity , Humans
Am J Trop Med Hyg ; 105(2): 449-453, 2021 Jul 08.
Article in English | MEDLINE | ID: covidwho-1371040


COVID-19 first emerged in Wuhan, China, in December 2019. Since that time, the frequency of bacterial and fungal coinfections has been continuously increasing. Although invasive pulmonary aspergillosis is being increasingly recognized in association with COVID-19, there is limited information regarding COVID-19-associated mucormycosis. We describe a 50-year-old woman with uncontrolled diabetes who received systemic corticosteroids and remdesevir during her admission for COVID-19. A few days after discharge, the patient was readmitted because of facial swelling and numbness, and a diagnosis of COVID-19-associated rhinosinusitis mucormycosis caused by Rhizopus arrhizus (formerly called Rhizopus oryzae) was confirmed with sequencing of the internal transcribed spacer region of the ribosomal DNA. This report aimed to address the importance of short-term follow-up for COVID-19 patients who have received systemic corticosteroids, particularly those with predisposing conditions, because early detection and prompt, aggressive treatment are essential for the management of invasive fungal infections.

Adrenal Cortex Hormones/therapeutic use , COVID-19/complications , COVID-19/drug therapy , Rhinitis/etiology , Rhizopus oryzae/pathogenicity , SARS-CoV-2/drug effects , Sinusitis/etiology , Adrenal Cortex Hormones/administration & dosage , Adrenal Cortex Hormones/adverse effects , DNA, Fungal/genetics , DNA, Ribosomal Spacer/genetics , Diabetes Mellitus , Fatal Outcome , Female , Humans , Invasive Fungal Infections/etiology , Middle Aged , Mucormycosis , Rhinitis/diagnosis , Rhinitis/microbiology , Sinusitis/diagnosis , Sinusitis/microbiology
Clin Infect Dis ; 72(Suppl 2): S95-S101, 2021 03 12.
Article in English | MEDLINE | ID: covidwho-1338678


Aspergillus polymerase chain reaction testing of blood and respiratory samples has recently been included in the second revision of the EORTC/MSGERC definitions for classifying invasive fungal disease. This is a result of considerable efforts to standardize methodology, the availability of commercial assays and external quality control programs, and additional clinical validation. This supporting article provides both clinical and technical justifications for its inclusion while also summarizing recent advances and likely future developments in the molecular diagnosis of invasive aspergillosis.

Aspergillosis , Invasive Fungal Infections , Aspergillosis/diagnosis , Aspergillus/genetics , DNA, Fungal/genetics , Humans , Invasive Fungal Infections/diagnosis , Polymerase Chain Reaction , Sensitivity and Specificity