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Biosens Bioelectron ; 171: 112753, 2021 Jan 01.
Article in English | MEDLINE | ID: covidwho-885210


A polyethyleneimine (PEI)-assisted copper in-situ growth (CISG) strategy was proposed as a controlled signal amplification strategy to enhance the sensitivity of gold nanoparticle-based lateral flow sensors (AuNP-LFS). The controlled signal amplification is achieved by introducing PEI as a structure-directing agent to regulate the thermodynamics of anisotropic Cu nanoshell growth on the AuNP surface, thus controlling shape and size of the resultant AuNP@Cu core-shell nanostructures and confining free reduction and self-nucleation of Cu2+ for improved reproducibility and decreased false positives. The PEI-CISG-enhanced AuNP-LFS showed ultrahigh sensitivities with the detection limits of 50 fg mL-1 for HIV-1 capsid p24 antigen and 6 CFU mL-1 for Escherichia coli O157:H7. We further demonstrated its clinical diagnostic efficacy by configuring PEI-CISG into a commercial AuNP-LFS detection kit for SARS-CoV-2 antibody detection. Altogether, this work provides a reliable signal amplification platform to dramatically enhance the sensitivity of AuNP-LFS for rapid and accurate diagnostics of various infectious diseases.

Biosensing Techniques/methods , Copper/chemistry , Coronavirus Infections/diagnosis , Escherichia coli Infections/diagnosis , Gold/chemistry , HIV Infections/diagnosis , Pneumonia, Viral/diagnosis , Betacoronavirus/isolation & purification , Biosensing Techniques/instrumentation , COVID-19 , COVID-19 Testing , Clinical Laboratory Techniques , Equipment Design , Escherichia coli O157/isolation & purification , HIV Core Protein p24/analysis , HIV-1/isolation & purification , Humans , Limit of Detection , Metal Nanoparticles/chemistry , Metal Nanoparticles/ultrastructure , Oxidation-Reduction , Pandemics , Polyethyleneimine/chemistry , Reagent Strips/analysis , SARS-CoV-2