Your browser doesn't support javascript.
Show: 20 | 50 | 100
Results 1 - 9 de 9
Filter
1.
Sensors (Basel) ; 21(17)2021 Aug 31.
Article in English | MEDLINE | ID: covidwho-1390737

ABSTRACT

While mRNA vaccines have been well-studied in vitro and in animals prior to their use in the human population during the Covid-19 pandemic, their exact mechanisms of inducing immunity are still being elucidated. The large-scale collection of data necessary to fully understand these mechanisms, and their variability across heterogeneous populations, requires rapid diagnostic tests that accurately measure the various biomarkers involved in the immune response following vaccination. Recently, our lab developed a novel "Disposable Photonics" platform for rapid, label-free, scalable diagnostics that utilizes photonic ring resonator sensor chips combined with plastic micropillar cards able to provide passive microfluidic flow. Here, we demonstrate the utility of this system in confirming the presence of SARS-CoV-2 spike protein in the serum of recently vaccinated subjects, as well as tracking a post-vaccination rise in anti-SARS-CoV-2 antibodies. A maximum concentration in SARS-CoV-2 spike protein was detected one day after vaccination and was reduced below detectable levels within 10 days. This highlights the applicability of our rapid photonic sensor platform for acquiring the data necessary to understand vaccine mechanisms on a large scale, as well as individual patient responses to SARS-CoV-2 mRNA vaccines.


Subject(s)
Biosensing Techniques , COVID-19 Vaccines , Spike Glycoprotein, Coronavirus , Antibodies, Viral , COVID-19 , Humans , Optics and Photonics , Pandemics , SARS-CoV-2 , Vaccination
2.
Adv Mater ; 33(23): e2006582, 2021 Jun.
Article in English | MEDLINE | ID: covidwho-1208338

ABSTRACT

Light guiding and manipulation in photonics have become ubiquitous in events ranging from everyday communications to complex robotics and nanomedicine. The speed and sensitivity of light-matter interactions offer unprecedented advantages in biomedical optics, data transmission, photomedicine, and detection of multi-scale phenomena. Recently, hydrogels have emerged as a promising candidate for interfacing photonics and bioengineering by combining their light-guiding properties with live tissue compatibility in optical, chemical, physiological, and mechanical dimensions. Herein, the latest progress over hydrogel photonics and its applications in guidance and manipulation of light is reviewed. Physics of guiding light through hydrogels and living tissues, and existing technical challenges in translating these tools into biomedical settings are discussed. A comprehensive and thorough overview of materials, fabrication protocols, and design architectures used in hydrogel photonics is provided. Finally, recent examples of applying structures such as hydrogel optical fibers, living photonic constructs, and their use as light-driven hydrogel robots, photomedicine tools, and organ-on-a-chip models are described. By providing a critical and selective evaluation of the field's status, this work sets a foundation for the next generation of hydrogel photonic research.


Subject(s)
Hydrogels/chemistry , Hydrogels/metabolism , Optics and Photonics/instrumentation , Animals , Biocompatible Materials/chemistry , Cell Culture Techniques , Drug Delivery Systems , Elastic Tissue/chemistry , Equipment and Supplies , Humans , Printing, Three-Dimensional , Surface Properties , Tissue Engineering
3.
Lab Chip ; 21(15): 2913-2921, 2021 08 07.
Article in English | MEDLINE | ID: covidwho-1279909

ABSTRACT

Decades of research have shown that biosensors using photonic circuits fabricated using CMOS processes can be highly sensitive, selective, and quantitative. Unfortunately, the cost of these sensors combined with the complexity of sample handling systems has limited the use of such sensors in clinical diagnostics. We present a new "disposable photonics" sensor platform in which rice-sized (1 × 4 mm) silicon nitride ring resonator sensor chips are paired with plastic micropillar fluidic cards for sample handling and optical detection. We demonstrate the utility of the platform in the context of detecting human antibodies to SARS-CoV-2, both in convalescent COVID-19 patients and for subjects undergoing vaccination. Given its ability to provide quantitative data on human samples in a simple, low-cost single-use format, we anticipate that this platform will find broad utility in clinical diagnostics for a broad range of assays.


Subject(s)
COVID-19 , Optics and Photonics , Biological Assay , COVID-19 Testing , Cost-Benefit Analysis , Humans , SARS-CoV-2
4.
Anal Chem ; 93(9): 4154-4159, 2021 03 09.
Article in English | MEDLINE | ID: covidwho-1108879

ABSTRACT

Chip-scale SARS-CoV-2 testing was demonstrated using silicon nitride (Si3N4) nanoslot fluidic waveguides to detect a tagged oligonucleotide with a coronavirus DNA sequence. The slot waveguides were fabricated using complementary metal-oxide-semiconductor (CMOS) fabrication processes, including multiscale lithography and selective reactive ion etching (RIE), forming femtoliter fluidic channels. Finite difference method (FDM) simulation was used to calculate the optical field distribution of the waveguide mode when the waveguide sensor was excited by transverse electric (TE) and transverse magnetic (TM) polarized light. For the TE polarization, a strong optical field was created in the slot region and its field intensity was 14× stronger than the evanescent sensing field from the TM polarization. The nanoscale confinement of the optical sensing field significantly enhanced the light-analyte interaction and improved the optical sensitivity. The sensitivity enhancement was experimentally demonstrated by measuring the polarization-dependent fluorescence emission from the tagged oligonucleotide. The photonic chips consisting of femtoliter Si3N4 waveguides provide a low-cost and high throughput platform for real-time virus identification, which is critical for point-of-care (PoC) diagnostic applications.


Subject(s)
COVID-19 Testing/methods , COVID-19/diagnosis , COVID-19/virology , DNA, Viral/analysis , Nanoparticles/chemistry , SARS-CoV-2/genetics , SARS-CoV-2/isolation & purification , Silicon Compounds/chemistry , Humans , Optics and Photonics , Point-of-Care Systems , Refractometry , Semiconductors , Sensitivity and Specificity
6.
Anal Chem ; 93(2): 828-833, 2021 01 19.
Article in English | MEDLINE | ID: covidwho-1065763

ABSTRACT

A variety of surface plasmon resonance (SPR) sensing devices have been extensively used in biochemical detection for their characteristics of label-free, highly sensitive, and faster detecting. Among them, the spectrum-based SPR sensing devices have offered us great advantages in high-throughput sensing due to their large dynamic range and the possibility of detection resolution similar to that offered by angle interrogation. This paper demonstrates a spectrum-based SPR imaging sensing system with fast wavelength scanning capability achieved by an acousto-optic tunable filter (AOTF) and a low-cost and speckle-free halogen lamp implemented as the SPR excitation source. Especially, we developed a novel four-parameter-based spectral curve readjusting (4-PSCR) method for data processing, which offered us a faster and more accurate spectral data curve fitting process than the traditional polynomial fitting method. With the configuration, we have also conducted an SPR high-throughput detection of the novel coronavirus (COVID-19) spike protein, proving its application possibility in the screening of COVID-19 with high accuracy. We believe that the higher sensitivity and accuracy of the system have made it readily used in biochemical imaging and detecting applications.


Subject(s)
Spike Glycoprotein, Coronavirus/analysis , Surface Plasmon Resonance/methods , Algorithms , COVID-19/diagnosis , COVID-19/virology , Humans , Limit of Detection , Optics and Photonics , SARS-CoV-2/isolation & purification , SARS-CoV-2/metabolism , Spike Glycoprotein, Coronavirus/metabolism , Surface Plasmon Resonance/instrumentation , Temperature
7.
Anal Chem ; 93(2): 828-833, 2021 01 19.
Article in English | MEDLINE | ID: covidwho-977246

ABSTRACT

A variety of surface plasmon resonance (SPR) sensing devices have been extensively used in biochemical detection for their characteristics of label-free, highly sensitive, and faster detecting. Among them, the spectrum-based SPR sensing devices have offered us great advantages in high-throughput sensing due to their large dynamic range and the possibility of detection resolution similar to that offered by angle interrogation. This paper demonstrates a spectrum-based SPR imaging sensing system with fast wavelength scanning capability achieved by an acousto-optic tunable filter (AOTF) and a low-cost and speckle-free halogen lamp implemented as the SPR excitation source. Especially, we developed a novel four-parameter-based spectral curve readjusting (4-PSCR) method for data processing, which offered us a faster and more accurate spectral data curve fitting process than the traditional polynomial fitting method. With the configuration, we have also conducted an SPR high-throughput detection of the novel coronavirus (COVID-19) spike protein, proving its application possibility in the screening of COVID-19 with high accuracy. We believe that the higher sensitivity and accuracy of the system have made it readily used in biochemical imaging and detecting applications.


Subject(s)
Spike Glycoprotein, Coronavirus/analysis , Surface Plasmon Resonance/methods , Algorithms , COVID-19/diagnosis , COVID-19/virology , Humans , Limit of Detection , Optics and Photonics , SARS-CoV-2/isolation & purification , SARS-CoV-2/metabolism , Spike Glycoprotein, Coronavirus/metabolism , Surface Plasmon Resonance/instrumentation , Temperature
8.
J Biophotonics ; 14(3): e202000338, 2021 03.
Article in English | MEDLINE | ID: covidwho-908749

ABSTRACT

The appearance of antibodies in blood is a critical signal to suggest the infection. A rapid and accurate detection method for the antibody is significant to the disease diagnosis, especially for the epidemic. To this end, a highly sensitive whispering-gallery-mode (WGM) optical testing kit is designed and fabricated for detecting the specific immunoglobulin antibodies. The key component of the kit is a silica self-assembled microsphere decorated with the nucleocapsid proteins (N-proteins) of the SARS-CoV-2 virus. After the N-protein antibody immunoglobulin G (N-IgG) and immunoglobulin M (N-IgM) solutions being injected into the kit, the WGM red-shifts due to the antigen-antibody reaction. The wavelength displacement rates are proportional to the concentrations of these two antibodies from 1 to 100 µg/mL. A good specificity of the kit is demonstrated by the nonspecific human immunoglobulin G (H-IgG) and immunoglobulin M (H-IgM).


Subject(s)
Antibodies, Viral/immunology , COVID-19 Serological Testing , COVID-19/diagnosis , Microspheres , Silicon Dioxide/chemistry , Antigens/immunology , Biosensing Techniques , COVID-19/immunology , Coronavirus Nucleocapsid Proteins/immunology , Epidemics , Humans , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Optics and Photonics , Phosphoproteins/immunology , Polymethyl Methacrylate/chemistry , SARS-CoV-2 , Silanes
9.
Opt Lett ; 45(19): 5428-5431, 2020 Oct 01.
Article in English | MEDLINE | ID: covidwho-810278

ABSTRACT

We show that waveguide sensors can enable a quantitative characterization of coronavirus spike glycoprotein-host-receptor binding-the process whereby coronaviruses enter human cells, causing disease. We demonstrate that such sensors can help quantify and eventually understand kinetic and thermodynamic properties of viruses that control their affinity to targeted cells, which is known to significantly vary in the course of virus evolution, e.g., from SARS-CoV to SARS-CoV-2, making the development of virus-specific drugs and vaccine difficult. With the binding rate constants and thermodynamic parameters as suggested by the latest SARS-CoV-2 research, optical sensors of SARS-CoV-2 spike protein-receptor binding may be within sight.


Subject(s)
Betacoronavirus , Biosensing Techniques , Coronavirus Infections , Optics and Photonics/instrumentation , Pandemics , Peptidyl-Dipeptidase A/metabolism , Pneumonia, Viral , Receptors, Virus/metabolism , Spike Glycoprotein, Coronavirus/metabolism , Angiotensin-Converting Enzyme 2 , Binding Sites , COVID-19 , Humans , Protein Binding/physiology , SARS-CoV-2
SELECTION OF CITATIONS
SEARCH DETAIL
...