A paper-based device for integrated high-throughput COVID-19 diagnostic tests

ABSTRACT

Novel microfluidic paper-based analytical devices (μPADs) were developed for qualitatively detecting biological pathogens. 32 reaction units for parallelized nucleic acid analysis were built on a single device. Also, an integrated system including modules of on-chip heating, temperature control, ultraviolet illumination and nucleic acid detection were designed and constructed. We first demonstrated the performance of the devices by detecting synthesized templates using Eiken loop-mediated isothermal amplification (LAMP) kit. Furthermore, the utility of the devices, system and protocols were verified by clinical tests. Multiple samples from COVID-19 cases were concurrently identified within 15 minutes using TianDZ RT-LAMP kit. © 2020 CBMS-0001