3D printed capillaric chip for instrumentation-free, rapid, and quantitative covid-19 serological testing using saliva

ABSTRACT

Our group developed a capillary-driven microfluidic technology called Domino Capillaric Circuits (DCC) that enables instrumentation-free, autonomous, preprogrammed sequential delivery of liquids, and that thus predisposed for rapid point-of-care diagnostics. Here, we present a capillaric chip, made by 3D printing, that automates reagent metering and sequential delivery of sample and multiple solutions to a lateral flow zone including rinsing and enzymatic amplification, and apply it to the quantification of SARS-CoV-2-specific antibodies response. Once loaded, sample addition by the user triggers all the fluidic operations, replicating the multiple incubation and washing steps common to ELISA and producing a colorimetric result visible by eye and quantifiable based on common imaging. DCCs might be further developed into fully integrated POC tests to address ongoing and emerging needs. © 2020 CBMS-0001