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Von Willebrand factor with increased binding capacity is associated with reduced platelet aggregation but enhanced agglutination in COVID-19 patients: another COVID-19 paradox?
Ruberto, Franco; Chistolini, Antonio; Curreli, Mariaignazia; Frati, Giacomo; Marullo, Antonino G M; Biondi-Zoccai, Giuseppe; Mancone, Massimo; Sciarretta, Sebastiano; Miraldi, Fabio; Alessandri, Francesco; Ceccarelli, Giancarlo; Barone, Francesco; Santoro, Cristina; Alvaro, Domenico; Pugliese, Francesco; Pulcinelli, Fabio M.
  • Ruberto F; Department of General Surgery, Surgical Specialities and Organ Transplantation "Paride Stefanini" Sapienza University of Rome, Rome, Italy.
  • Chistolini A; Hematology, Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.
  • Curreli M; Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy.
  • Frati G; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Latina, Italy.
  • Marullo AGM; IRCCS NEUROMED, Pozzilli, Italy.
  • Biondi-Zoccai G; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Latina, Italy.
  • Mancone M; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Latina, Italy.
  • Sciarretta S; Mediterranea Cardiocentro, Napoli, Italy.
  • Miraldi F; Department of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, Sapienza University of Rome, Rome, Italy.
  • Alessandri F; Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Latina, Italy.
  • Ceccarelli G; IRCCS NEUROMED, Pozzilli, Italy.
  • Barone F; Department of Clinical, Internal Medicine, Anesthesiology and Cardiovascular Sciences, Sapienza University of Rome, Rome, Italy.
  • Santoro C; Department of General Surgery, Surgical Specialities and Organ Transplantation "Paride Stefanini" Sapienza University of Rome, Rome, Italy.
  • Alvaro D; Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
  • Pugliese F; Hematology, Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.
  • Pulcinelli FM; Hematology, Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.
J Thromb Thrombolysis ; 52(1): 105-110, 2021 Jul.
Article in English | MEDLINE | ID: covidwho-1002136
Semantic information from SemMedBD (by NLM)
1. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
2. agglutination PROCESS_OF Patients
Subject
agglutination
Predicate
PROCESS_OF
Object
Patients
3. Blood Platelets LOCATION_OF IMPACT gene
Subject
Blood Platelets
Predicate
LOCATION_OF
Object
IMPACT gene
4. von Willebrand factor TREATS Cohort
Subject
von Willebrand factor
Predicate
TREATS
Object
Cohort
5. agglutination PROCESS_OF Blood Platelets
Subject
agglutination
Predicate
PROCESS_OF
Object
Blood Platelets
6. Platelet PROCESS_OF C0030705
Subject
Platelet
Predicate
PROCESS_OF
Object
C0030705
7. Ristocetin STIMULATES agglutination
Subject
Ristocetin
Predicate
STIMULATES
Object
agglutination
8. Platelet aggregation PROCESS_OF Patients
Subject
Platelet aggregation
Predicate
PROCESS_OF
Object
Patients
9. von Willebrand factor STIMULATES agglutination
Subject
von Willebrand factor
Predicate
STIMULATES
Object
agglutination
10. von Willebrand factor PREDISPOSES Complication
Subject
von Willebrand factor
Predicate
PREDISPOSES
Object
Complication
11. Platelet adhesio PROCESS_OF C0030705
Subject
Platelet adhesio
Predicate
PROCESS_OF
Object
C0030705
12. COVID-19 PROCESS_OF Patients
Subject
COVID-19
Predicate
PROCESS_OF
Object
Patients
13. agglutination PROCESS_OF Patients
Subject
agglutination
Predicate
PROCESS_OF
Object
Patients
14. Blood Platelets LOCATION_OF IMPACT gene
Subject
Blood Platelets
Predicate
LOCATION_OF
Object
IMPACT gene
15. von Willebrand factor TREATS Cohort
Subject
von Willebrand factor
Predicate
TREATS
Object
Cohort
16. agglutination PROCESS_OF Blood Platelets
Subject
agglutination
Predicate
PROCESS_OF
Object
Blood Platelets
17. Platelets, CTCAE PROCESS_OF Patients
Subject
Platelets, CTCAE
Predicate
PROCESS_OF
Object
Patients
18. Ristocetin STIMULATES agglutination
Subject
Ristocetin
Predicate
STIMULATES
Object
agglutination
19. Platelet aggregation PROCESS_OF Patients
Subject
Platelet aggregation
Predicate
PROCESS_OF
Object
Patients
20. von Willebrand factor STIMULATES agglutination
Subject
von Willebrand factor
Predicate
STIMULATES
Object
agglutination
21. von Willebrand factor PREDISPOSES Complication
Subject
von Willebrand factor
Predicate
PREDISPOSES
Object
Complication
22. Platelet adhesion, function PROCESS_OF Patients
Subject
Platelet adhesion, function
Predicate
PROCESS_OF
Object
Patients
ABSTRACT
Patients with Coronavirus-associated disease-2019 (COVID-19) display alterations of the hemostatic system and the presence of a prothrombotic status frequently leading to vascular complications. However, the impact of COVID-19 on platelet activity, aggregation and agglutination still needs to be clarified. We measured total levels of von Willebrand factor (vWF) and vWF binding to the platelet glycoprotein (Gp) complex (GPIb-IX-V), in a cohort of COVID-19 patients admitted to the intensive care unit of our Institution. Moreover, we evaluated platelet aggregation in response to agonists (ADP, collagen, arachidonic acid) and platelet agglutination in response to ristocetin. We found that levels of vWF antigen and the active form of vWF binding to platelets (vWFRCo), were markedly increased in these patients. These results were associated with higher agglutination rates induced by ristocetin, thereby indirectly indicating an increased capability of vWF to bind to platelets. Conversely, we found that platelet aggregation in response to both ADP and collagen was lower in COVID-19 patients compared to healthy volunteers. This study shows that COVID-19 is associated with increased vWF-induced platelet agglutination but reduced platelet responsivity to aggregation stimuli. Our findings have translational relevance since platelet adhesion to vWF may represent a marker to predict possible complications and better delineate therapeutic strategies in COVID-19 patients.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Blood Platelets / Von Willebrand Factor / Platelet Aggregation / COVID-19 Subject: Blood Platelets / Von Willebrand Factor / Platelet Aggregation / COVID-19 Type of study: Diagnostic study / Prognostic study / Risk factors Language: English Journal: J Thromb Thrombolysis Clinical aspect: Etiology / Prediction / Prognosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Blood Platelets / Von Willebrand Factor / Platelet Aggregation / COVID-19 Subject: Blood Platelets / Von Willebrand Factor / Platelet Aggregation / COVID-19 Type of study: Diagnostic study / Prognostic study / Risk factors Language: English Journal: J Thromb Thrombolysis Clinical aspect: Etiology / Prediction / Prognosis Year: 2021
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