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A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2.
Maricic, Tomislav; Nickel, Olaf; Aximu-Petri, Ayinuer; Essel, Elena; Gansauge, Marie; Kanis, Philipp; Macak, Dominik; Richter, Julia; Riesenberg, Stephan; Bokelmann, Lukas; Zeberg, Hugo; Meyer, Matthias; Borte, Stephan; Pääbo, Svante.
  • Maricic T; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Nickel O; Department of Laboratory Medicine, Hospital St. Georg, Leipzig, Germany.
  • Aximu-Petri A; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Essel E; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Gansauge M; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Kanis P; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Macak D; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Richter J; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Riesenberg S; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Bokelmann L; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Zeberg H; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Meyer M; Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
  • Borte S; Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.
  • Pääbo S; Department of Laboratory Medicine, Hospital St. Georg, Leipzig, Germany.
PLoS One ; 15(12): e0244824, 2020.
Article in English | MEDLINE | ID: covidwho-1004472
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ABSTRACT
SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages ("mouthwashes"). This method detects individuals with large viral loads (Ct≤29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mass Screening / Real-Time Polymerase Chain Reaction / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2020 Document Type: Article Affiliation country: Journal.pone.0244824

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Mass Screening / Real-Time Polymerase Chain Reaction / COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: PLoS One Journal subject: Science / Medicine Year: 2020 Document Type: Article Affiliation country: Journal.pone.0244824