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Dose-dependent response to infection with SARS-CoV-2 in the ferret model and evidence of protective immunity.
Ryan, Kathryn A; Bewley, Kevin R; Fotheringham, Susan A; Slack, Gillian S; Brown, Phillip; Hall, Yper; Wand, Nadina I; Marriott, Anthony C; Cavell, Breeze E; Tree, Julia A; Allen, Lauren; Aram, Marilyn J; Bean, Thomas J; Brunt, Emily; Buttigieg, Karen R; Carter, Daniel P; Cobb, Rebecca; Coombes, Naomi S; Findlay-Wilson, Steve J; Godwin, Kerry J; Gooch, Karen E; Gouriet, Jade; Halkerston, Rachel; Harris, Debbie J; Hender, Thomas H; Humphries, Holly E; Hunter, Laura; Ho, Catherine M K; Kennard, Chelsea L; Leung, Stephanie; Longet, Stephanie; Ngabo, Didier; Osman, Karen L; Paterson, Jemma; Penn, Elizabeth J; Pullan, Steven T; Rayner, Emma; Skinner, Oliver; Steeds, Kimberley; Taylor, Irene; Tipton, Tom; Thomas, Stephen; Turner, Carrie; Watson, Robert J; Wiblin, Nathan R; Charlton, Sue; Hallis, Bassam; Hiscox, Julian A; Funnell, Simon; Dennis, Mike J.
  • Ryan KA; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Bewley KR; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Fotheringham SA; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Slack GS; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Brown P; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Hall Y; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Wand NI; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Marriott AC; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Cavell BE; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Tree JA; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Allen L; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Aram MJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Bean TJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Brunt E; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Buttigieg KR; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Carter DP; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Cobb R; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Coombes NS; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Findlay-Wilson SJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Godwin KJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Gooch KE; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Gouriet J; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Halkerston R; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Harris DJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Hender TH; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Humphries HE; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Hunter L; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Ho CMK; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Kennard CL; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Leung S; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Longet S; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Ngabo D; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Osman KL; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Paterson J; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Penn EJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Pullan ST; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Rayner E; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Skinner O; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Steeds K; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Taylor I; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Tipton T; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Thomas S; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Turner C; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Watson RJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Wiblin NR; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Charlton S; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Hallis B; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Hiscox JA; Institute of Infection and Global Health, University of Liverpool, Liverpool, L69 2BE, UK.
  • Funnell S; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
  • Dennis MJ; National Infection Service, Public Health England (PHE), Porton Down, Salisbury, Wiltshire, SP4 0JG, UK.
Nat Commun ; 12(1): 81, 2021 01 04.
Article in English | MEDLINE | ID: covidwho-1007628
ABSTRACT
There is a vital need for authentic COVID-19 animal models to enable the pre-clinical evaluation of candidate vaccines and therapeutics. Here we report a dose titration study of SARS-CoV-2 in the ferret model. After a high (5 × 106 pfu) and medium (5 × 104 pfu) dose of virus is delivered, intranasally, viral RNA shedding in the upper respiratory tract (URT) is observed in 6/6 animals, however, only 1/6 ferrets show similar signs after low dose (5 × 102 pfu) challenge. Following sequential culls pathological signs of mild multifocal bronchopneumonia in approximately 5-15% of the lung is seen on day 3, in high and medium dosed groups. Ferrets re-challenged, after virus shedding ceased, are fully protected from acute lung pathology. The endpoints of URT viral RNA replication & distinct lung pathology are observed most consistently in the high dose group. This ferret model of SARS-CoV-2 infection presents a mild clinical disease.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Disease Models, Animal / Ferrets / SARS-CoV-2 / COVID-19 Subject: Disease Models, Animal / Ferrets / SARS-CoV-2 / COVID-19 Type of study: Evidence synthesis / Prognostic study Language: English Journal: Nat Commun Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Document Type: Article Main subject: Disease Models, Animal / Ferrets / SARS-CoV-2 / COVID-19 Subject: Disease Models, Animal / Ferrets / SARS-CoV-2 / COVID-19 Type of study: Evidence synthesis / Prognostic study Language: English Journal: Nat Commun Year: 2021
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