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Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins.
Ahmadivand, Arash; Gerislioglu, Burak; Ramezani, Zeinab; Kaushik, Ajeet; Manickam, Pandiaraj; Ghoreishi, S Amir.
  • Ahmadivand A; Department of Electrical and Computer Engineering, Rice University, 6100 Main St, Houston, TX, 77005, United States; Metamaterial Technologies Inc, Pleasanton, CA, 94588, United States. Electronic address: arash.ahmadivand@metamaterial.com.
  • Gerislioglu B; Department of Physics and Astronomy, Rice University, 6100 Main St, Houston, TX, 77005, United States.
  • Ramezani Z; Department of Electrical and Computer Engineering, Northeastern University, Boston, MA, 02115, United States.
  • Kaushik A; NanoBioTech Laboratory, Department of Natural Sciences, Division of Sciences, Art, & Mathematics, Florida Polytechnic University, Lakeland, FL, 33805, United States.
  • Manickam P; Electrodics and Electrocatalysis Division, CSIR-Central Electrochemical Research Institute (CECRI), Karaikudi, 630 003, Tamil Nadu, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201 002, Uttar Pradesh, India.
  • Ghoreishi SA; Faculty of Electrical & Computer Engineering, Science and Research Branch, Islamic Azad University of Tehran, Tehran, Iran; Department of Electrical Engineering, Varamin (Pishva) Branch Islamic Azad University, Varamin, Iran.
Biosens Bioelectron ; 177: 112971, 2021 Apr 01.
Article in English | MEDLINE | ID: covidwho-1009326
ABSTRACT
Effective and efficient management of human betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 virus infection i.e., COVID-19 pandemic, required sensitive and selective sensors with short sample-to-result durations for performing desired diagnostics. In this direction, one appropriate alternative approach to detect SARS-CoV-2 virus protein at low level i.e., femtomolar (fM) is exploring plasmonic metasensor technology for COVID-19 diagnostics, which offers exquisite opportunities in advanced healthcare programs, and modern clinical diagnostics. The intrinsic merits of plasmonic metasensors stem from their capability to squeeze electromagnetic fields, simultaneously in frequency, time, and space. However, the detection of low-molecular weight biomolecules at low densities is a typical drawback of conventional metasensors that has recently been addressed using toroidal metasurface technology. This research is focused on the fabrication of a miniaturized plasmonic immunosensor based on toroidal electrodynamics concept that can sustain robustly confined plasmonic modes with ultranarrow lineshapes in the terahertz (THz) frequencies. By exciting toroidal dipole mode using our quasi-infinite metasurface and a judiciously optimized protocol based on functionalized gold nanoparticles (AuNPs) conjugated with the specific monoclonal antibody specific to spike protein (S1) of SARS-CoV-2 virus onto the metasurface, the resonance shifts for diverse concentrations of the spike protein are monitored. Possessing molecular weight around ~76 kDa allowed to detect the presence of SARS-CoV-2 virus protein with significantly low as limit of detection (LoD) was achieved as ~4.2 fM. We envisage that outcomes of this research will pave the way toward the use of toroidal metasensors as practical technologies for rapid and precise screening of SARS-CoV-2 virus carriers, symptomatic or asymptomatic, and spike proteins in hospitals, clinics, laboratories, and site of infection.
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Full text: Available Collection: International databases Database: MEDLINE Type: Article Main subject: Biosensing Techniques / Antibodies, Immobilized / Spike Glycoprotein, Coronavirus Subject: Biosensing Techniques / Antibodies, Immobilized / Spike Glycoprotein, Coronavirus Type of study: Diagnostic study Language: English Journal: Biosens Bioelectron Clinical aspect: Diagnosis Year: 2021

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Full text: Available Collection: International databases Database: MEDLINE Type: Article Main subject: Biosensing Techniques / Antibodies, Immobilized / Spike Glycoprotein, Coronavirus Subject: Biosensing Techniques / Antibodies, Immobilized / Spike Glycoprotein, Coronavirus Type of study: Diagnostic study Language: English Journal: Biosens Bioelectron Clinical aspect: Diagnosis Year: 2021