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Cold sensitivity of the SARS-CoV-2 spike ectodomain.
Edwards, Robert J; Mansouri, Katayoun; Stalls, Victoria; Manne, Kartik; Watts, Brian; Parks, Rob; Janowska, Katarzyna; Gobeil, Sophie M C; Kopp, Megan; Li, Dapeng; Lu, Xiaozhi; Mu, Zekun; Deyton, Margaret; Oguin, Thomas H; Sprenz, Jordan; Williams, Wilton; Saunders, Kevin O; Montefiori, David; Sempowski, Gregory D; Henderson, Rory; Munir Alam, S; Haynes, Barton F; Acharya, Priyamvada.
  • Edwards RJ; Duke Human Vaccine Institute, Durham, NC, USA. rj.edwards@duke.edu.
  • Mansouri K; Department of Medicine, Duke University, Durham, NC, USA. rj.edwards@duke.edu.
  • Stalls V; Duke Human Vaccine Institute, Durham, NC, USA.
  • Manne K; Duke Human Vaccine Institute, Durham, NC, USA.
  • Watts B; Duke Human Vaccine Institute, Durham, NC, USA.
  • Parks R; Duke Human Vaccine Institute, Durham, NC, USA.
  • Janowska K; Duke Human Vaccine Institute, Durham, NC, USA.
  • Gobeil SMC; Duke Human Vaccine Institute, Durham, NC, USA.
  • Kopp M; Duke Human Vaccine Institute, Durham, NC, USA.
  • Li D; Duke Human Vaccine Institute, Durham, NC, USA.
  • Lu X; Duke Human Vaccine Institute, Durham, NC, USA.
  • Mu Z; Duke Human Vaccine Institute, Durham, NC, USA.
  • Deyton M; Duke Human Vaccine Institute, Durham, NC, USA.
  • Oguin TH; Duke Human Vaccine Institute, Durham, NC, USA.
  • Sprenz J; Duke Human Vaccine Institute, Durham, NC, USA.
  • Williams W; Duke Human Vaccine Institute, Durham, NC, USA.
  • Saunders KO; Duke Human Vaccine Institute, Durham, NC, USA.
  • Montefiori D; Department of Medicine, Duke University, Durham, NC, USA.
  • Sempowski GD; Duke Human Vaccine Institute, Durham, NC, USA.
  • Henderson R; Department of Surgery, Duke University, Durham, NC, USA.
  • Munir Alam S; Duke Human Vaccine Institute, Durham, NC, USA.
  • Haynes BF; Department of Surgery, Duke University, Durham, NC, USA.
  • Acharya P; Duke Human Vaccine Institute, Durham, NC, USA.
Nat Struct Mol Biol ; 28(2): 128-131, 2021 02.
Article in English | MEDLINE | ID: covidwho-1010060
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
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ABSTRACT
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible 'up' or receptor-inaccessible 'down' states. Here we report that the commonly used stabilized S ectodomain construct '2P' is sensitive to cold temperatures, and this cold sensitivity is abrogated in a 'down' state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus Topics: Vaccines Limits: Humans Language: English Journal: Nat Struct Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: S41594-020-00547-5

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus Topics: Vaccines Limits: Humans Language: English Journal: Nat Struct Mol Biol Journal subject: Molecular Biology Year: 2021 Document Type: Article Affiliation country: S41594-020-00547-5