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Assessment of inactivation procedures for SARS-CoV-2.
Auerswald, Heidi; Yann, Sokhoun; Dul, Sokha; In, Saraden; Dussart, Philippe; Martin, Nicholas J; Karlsson, Erik A; Garcia-Rivera, Jose A.
  • Auerswald H; Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
  • Yann S; Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
  • Dul S; Naval Medical Research Unit TWO, Phnom Penh, Cambodia.
  • In S; Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
  • Dussart P; Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
  • Martin NJ; Naval Medical Research Unit TWO, Singapore.
  • Karlsson EA; Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
  • Garcia-Rivera JA; Naval Medical Research Unit TWO, Phnom Penh, Cambodia.
J Gen Virol ; 102(3)2021 03.
Article in English | MEDLINE | ID: covidwho-1015423
Preprint
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ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), presents a challenge to laboratorians and healthcare workers around the world. Handling of biological samples from individuals infected with the SARS-CoV-2 virus requires strict biosafety measures. Within the laboratory, non-propagative work with samples containing the virus requires, at minimum, Biosafety Level-2 (BSL-2) techniques and facilities. Therefore, handling of SARS-CoV-2 samples remains a major concern in areas and conditions where biosafety for specimen handling is difficult to maintain, such as in rural laboratories or austere field testing sites. Inactivation through physical or chemical means can reduce the risk of handling live virus and increase testing ability especially in low-resource settings due to easier and faster sample processing. Herein we assess several chemical and physical inactivation techniques employed against SARS-CoV-2 isolates from Cambodia. This data demonstrates that all chemical (AVL, inactivating sample buffer and formaldehyde) and heat-treatment (56 and 98 °C) methods tested completely inactivated viral loads of up to 5 log10.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Specimen Handling / Containment of Biohazards / Virus Inactivation / SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Animals / Humans Country/Region as subject: Asia Language: English Year: 2021 Document Type: Article Affiliation country: Jgv.0.001539

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Specimen Handling / Containment of Biohazards / Virus Inactivation / SARS-CoV-2 / COVID-19 Type of study: Prognostic study Limits: Animals / Humans Country/Region as subject: Asia Language: English Year: 2021 Document Type: Article Affiliation country: Jgv.0.001539