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The N gene of SARS-CoV-2 was the main positive component in repositive samples from a cohort of COVID-19 patients in Wuhan, China.
Zhang, Xuzhao; Li, Min; Zhang, Bin; Chen, Tao; Lv, Dong; Xia, Pengfei; Sun, Zhuanyi; Shentu, Xiaoyan; Chen, Haiyan; Li, Libin; Qian, Wenbin.
  • Zhang X; Department of Hematology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China. Electronic address: zxzzju@zju.edu
  • Li M; Department of Neurology Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Zhang B; Department of Respiratory Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Chen T; Department of Neurology Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Lv D; Department of Respiratory Medicine, Shangyu People's Hospital, Shangyu 312300, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Xia P; Department of Respiratory Medicine, Lanxi People's Hospital, Lanxi 321100, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Sun Z; Department of Hematology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Shentu X; Department of Dermatology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Chen H; Department of Hematology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Li L; Department of Surgery Intensive Care Unit, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China; Aid Hubei Medical Team, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
  • Qian W; Department of Hematology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, China.
Clin Chim Acta ; 511: 291-297, 2020 Dec.
Article in English | MEDLINE | ID: covidwho-1023490
ABSTRACT

BACKGROUND:

Repositivity of SARS-CoV-2 nucleic acid in discharged COVID-19 patients was reported recently. However, the characteristics of repositive results are still not well understood, leading to a lack of effective monitoring strategies.

METHODS:

In the present study, a total of 59 COVID-19 patients were enrolled, and the characteristics of the repositive samples were analyzed.

RESULTS:

The repositive rate in this cohort was 15.79%. The N gene was the main target gene that was positive in the repositive results as well as in the last positive results of all patients. The median duration from diagnosis to the last positive test was 20 days (IQR, 16-31 days), and the longest duration was 40 days. Repositivity was only observed in IgM single- or both IgM- and IgG-positive patients, instead of IgG single-positive patients.

CONCLUSIONS:

There was a significant proportion of repositives in the recovered COVID-19 patients, and increasing the required number of negatives for consecutive nucleic acid tests may reduce the incidence of repositives. The recommended monitoring strategy for repositivity is monitoring the N gene in IgM-positive patients. This can ensure high sensitivity while reducing the time and cost of nucleic acid detection.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Real-Time Polymerase Chain Reaction / SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Aged / Female / Humans / Male / Middle aged Country/Region as subject: Asia Language: English Journal: Clin Chim Acta Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Real-Time Polymerase Chain Reaction / SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Aged / Female / Humans / Male / Middle aged Country/Region as subject: Asia Language: English Journal: Clin Chim Acta Year: 2020 Document Type: Article