Performance of a multiplex polymerase chain reaction panel for identifying bacterial pathogens causing pneumonia in critically ill patients with COVID-19.
Diagn Microbiol Infect Dis
; 99(1): 115183, 2021 Jan.
Article
in English
| MEDLINE | ID: covidwho-1023526
ABSTRACT
The FilmArray® Pneumonia Plus (FA-PP) panel can provide rapid identifications and semiquantitative results for many pathogens. We performed a prospective single-center study in 43 critically ill patients with coronavirus disease 2019 (COVID-19) in which we performed 96 FA-PP tests and cultures of blind bronchoalveolar lavage (BBAL). FA-PP detected 1 or more pathogens in 32% (31/96 of samples), whereas culture methods detected at least 1 pathogen in 35% (34/96 of samples). The most prevalent bacteria detected were Pseudomonas aeruginosa (nâ¯=â¯14) and Staphylococcus aureus (nâ¯=â¯11) on both FA-PP and culture. The FA-PP results from BBAL in critically ill patients with COVID-19 were consistent with bacterial culture findings for bacteria present in the FA-PP panel, showing sensitivity, specificity, and positive and negative predictive value of 95%, 99%, 82%, and 100%, respectively. Median turnaround time for FA-PP was 5.5â¯h, which was significantly shorter than for standard culture (26â¯h) and antimicrobial susceptibility testing results (57â¯h).
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Bacteria
/
Bacteriological Techniques
/
Pneumonia, Bacterial
/
Multiplex Polymerase Chain Reaction
/
COVID-19
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Topics:
Long Covid
Limits:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
English
Journal:
Diagn Microbiol Infect Dis
Year:
2021
Document Type:
Article
Affiliation country:
J.diagmicrobio.2020.115183
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