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Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples.
Eis-Hübinger, Anna M; Hönemann, Mario; Wenzel, Jürgen J; Berger, Annemarie; Widera, Marek; Schmidt, Barbara; Aldabbagh, Souhaib; Marx, Benjamin; Streeck, Hendrik; Ciesek, Sandra; Liebert, Uwe G; Huzly, Daniela; Hengel, Hartmut; Panning, Marcus.
  • Eis-Hübinger AM; Institute of Virology, University of Bonn, Faculty of Medicine, Bonn, Germany.
  • Hönemann M; Institute of Virology, Medical Faculty, University of Leipzig, Leipzig, Germany.
  • Wenzel JJ; Institute of Clinical Microbiology and Hygiene, Regensburg University Hospital, Regensburg, Germany.
  • Berger A; Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.
  • Widera M; Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.
  • Schmidt B; Institute of Clinical Microbiology and Hygiene, Regensburg University Hospital, Regensburg, Germany.
  • Aldabbagh S; Institute of Virology, University of Bonn, Faculty of Medicine, Bonn, Germany.
  • Marx B; Institute of Virology, University of Bonn, Faculty of Medicine, Bonn, Germany.
  • Streeck H; Institute of Virology, University of Bonn, Faculty of Medicine, Bonn, Germany.
  • Ciesek S; Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.
  • Liebert UG; Institute of Virology, Medical Faculty, University of Leipzig, Leipzig, Germany.
  • Huzly D; Institute of Virology, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • Hengel H; Institute of Virology, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • Panning M; Institute of Virology, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany. Electronic address: marcus.panning@uniklinik-freiburg.de.
J Clin Virol ; 127: 104381, 2020 06.
Article in English | MEDLINE | ID: covidwho-102384
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Semantic information from SemMedBD (by NLM)
1. 2019 novel coronavirus PROCESS_OF hospitalized patients
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
hospitalized patients
2. 2019 novel coronavirus PROCESS_OF hospitalized patients
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
hospitalized patients
ABSTRACT

BACKGROUND:

A novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in China in late 2019 and subsequently caused a pandemic. Surveillance is important to better appreciate this evolving pandemic and to longitudinally monitor the effectiveness of public health measures.

OBJECTIVES:

We aimed to provide a rapid, easy to establish and costeffective laboratory-based surveillance tool for SARS-CoV-2. STUDY

DESIGN:

We used minipools of RNA prepared from nucleic acid extractions of routine respiratory samples. We technically validated the assay and distributed the protocol within an informal network of five German university laboratories.

RESULTS:

We tested a total of 70 minipools resembling 700 samples shortly before the upsurge of cases in Germany from 17.02.2020 to 10.03.2020. One minipool reacted positive and after resolution one individual sample tested SARS-CoV-2 positive. This sample was from a hospitalized patient not suspected of having contracted SARS-CoV-2.

CONCLUSIONS:

Our approach of a laboratory-based surveillance for SARSCoV-2 using minipools proved its concept is easily adaptable and resource-saving. It might assist not only public health laboratories in SARS-CoV-2 surveillance.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / RNA, Viral / Coronavirus Infections / Reverse Transcriptase Polymerase Chain Reaction / Molecular Diagnostic Techniques / Epidemiological Monitoring Type of study: Diagnostic study / Observational study / Risk factors / Screening study Topics: Long Covid Limits: Humans Country/Region as subject: Europa Language: English Journal: J Clin Virol Journal subject: Virology Year: 2020 Document Type: Article Affiliation country: J.jcv.2020.104381

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pneumonia, Viral / RNA, Viral / Coronavirus Infections / Reverse Transcriptase Polymerase Chain Reaction / Molecular Diagnostic Techniques / Epidemiological Monitoring Type of study: Diagnostic study / Observational study / Risk factors / Screening study Topics: Long Covid Limits: Humans Country/Region as subject: Europa Language: English Journal: J Clin Virol Journal subject: Virology Year: 2020 Document Type: Article Affiliation country: J.jcv.2020.104381