Ultrastructural modifications induced by SARS-CoV-2 in Vero cells: a kinetic analysis of viral factory formation, viral particle morphogenesis and virion release.
Cell Mol Life Sci
; 78(7): 3565-3576, 2021 Apr.
Article
in English
| MEDLINE | ID: covidwho-1384325
ABSTRACT
Many studies on SARS-CoV-2 have been performed over short-time scale, but few have focused on the ultrastructural characteristics of infected cells. We used TEM to perform kinetic analysis of the ultrastructure of SARS-CoV-2-infected cells. Early infection events were characterized by the presence of clusters of single-membrane vesicles and stacks of membrane containing nuclear pores called annulate lamellae (AL). A large network of host cell-derived organelles transformed into virus factories was subsequently observed in the cells. As previously described for other RNA viruses, these replication factories consisted of double-membrane vesicles (DMVs) located close to the nucleus. Viruses released at the cell surface by exocytosis harbored the typical crown of spike proteins, but viral particles without spikes were also observed in intracellular compartments, possibly reflecting incorrect assembly or a cell degradation process.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Virus Replication
/
Virus Release
/
Viral Replication Compartments
/
SARS-CoV-2
Limits:
Animals
Language:
English
Journal:
Cell Mol Life Sci
Journal subject:
Molecular Biology
Year:
2021
Document Type:
Article
Affiliation country:
S00018-020-03745-Y
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