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Peripheral Blood Immune Profiling of Convalescent Plasma Donors Reveals Alterations in Specific Immune Subpopulations Even at 2 Months Post SARS-CoV-2 Infection.
Orologas-Stavrou, Nikolaos; Politou, Marianna; Rousakis, Pantelis; Kostopoulos, Ioannis V; Ntanasis-Stathopoulos, Ioannis; Jahaj, Edison; Tsiligkeridou, Eleni; Gavriatopoulou, Maria; Kastritis, Efstathios; Kotanidou, Anastasia; Dimopoulos, Meletios-Athanasios; Tsitsilonis, Ourania E; Terpos, Evangelos.
  • Orologas-Stavrou N; Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
  • Politou M; Hematology Laboratory-Blood Bank, Aretaieion Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.
  • Rousakis P; Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
  • Kostopoulos IV; Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
  • Ntanasis-Stathopoulos I; Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.
  • Jahaj E; First Department of Critical Care Medicine and Pulmonary Services, Evangelismos General Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece.
  • Tsiligkeridou E; Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.
  • Gavriatopoulou M; Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.
  • Kastritis E; Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.
  • Kotanidou A; First Department of Critical Care Medicine and Pulmonary Services, Evangelismos General Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece.
  • Dimopoulos MA; Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.
  • Tsitsilonis OE; Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.
  • Terpos E; Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.
Viruses ; 13(1)2020 12 25.
Article in English | MEDLINE | ID: covidwho-1043520
ABSTRACT
Immune profiling of patients with COVID-19 has shown that SARS-CoV-2 causes severe lymphocyte deficiencies (e.g., lymphopenia, decreased numbers, and exhaustion of T cells) and increased levels of pro-inflammatory monocytes. Peripheral blood (PB) samples from convalescent plasma (CP) donors, COVID-19 patients, and control subjects were analyzed by multiparametric flow cytometry, allowing the identification of a wide panel of immune cells, comprising lymphocytes (T, B, natural killer (NK) and NKT cells), monocytes, granulocytes, and their subsets. Compared to active COVID-19 patients, our results revealed that the immune profile of recovered donors was restored for most subpopulations. Nevertheless, even 2 months after recovery, CP donors still had reduced levels of CD4+ T and B cells, as well as granulocytes. CP donors with non-detectable levels of anti-SARS-CoV-2-specific antibodies in their serum were characterized by higher Th9 and Th17 cells, which were possibly expanded at the expense of Th2 humoral immunity. The most noticeable alterations were identified in previously hospitalized CP donors, who presented the lowest levels of CD8+ regulatory T cells, the highest levels of CD56+CD16- NKT cells, and a promotion of a Th17-type phenotype, which might be associated with a prolonged pro-inflammatory response. A longer follow-up of CP donors will eventually reveal the time needed for full recovery of their immune system competence.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plasma / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Cohort study / Prognostic study Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Year: 2020 Document Type: Article Affiliation country: V13010026

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Plasma / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Cohort study / Prognostic study Limits: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Year: 2020 Document Type: Article Affiliation country: V13010026