The porcine deltacoronavirus accessory protein NS6 is expressed in vivo and incorporated into virions.
Virology
; 556: 1-8, 2021 04.
Article
in English
| MEDLINE | ID: covidwho-1045103
ABSTRACT
Porcine deltacoronavirus (PDCoV) is one of the emerged coronaviruses posing a significant threat to the swine industry. Previous work showed the presence of a viral accessory protein NS6 in PDCoV-infected cells. In this study, we detected the expression of the NS6 protein in small intestinal tissues of PDCoV-infected piglets. In addition, SDS-PAGE and Western blot analysis of sucrose gradient-purified virions showed the presence of a 13-kDa NS6 protein. Further evidences of the presence of NS6 in the PDCoV virions were obtained by immunogold staining of purified virions with anti-NS6 antiserum, and by immunoprecipitation of NS6 from purified virions. Finally, the anti-NS6 antibody was not able to neutralize PDCoV in cultured cells. These data establish for the first time that the accessory protein NS6 is expressed during infection in vivo and incorporated into PDCoV virions.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Swine Diseases
/
Virion
/
Viral Nonstructural Proteins
/
Coronavirus Infections
/
Deltacoronavirus
Limits:
Animals
Language:
English
Journal:
Virology
Year:
2021
Document Type:
Article
Affiliation country:
J.virol.2021.01.011
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