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1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5'-UTR of SARS-CoV-2.
Schnieders, Robbin; Peter, Stephen A; Banijamali, Elnaz; Riad, Magdalena; Altincekic, Nadide; Bains, Jasleen Kaur; Ceylan, Betül; Fürtig, Boris; Grün, J Tassilo; Hengesbach, Martin; Hohmann, Katharina F; Hymon, Daniel; Knezic, Bozana; Oxenfarth, Andreas; Petzold, Katja; Qureshi, Nusrat S; Richter, Christian; Schlagnitweit, Judith; Schlundt, Andreas; Schwalbe, Harald; Stirnal, Elke; Sudakov, Alexey; Vögele, Jennifer; Wacker, Anna; Weigand, Julia E; Wirmer-Bartoschek, Julia; Wöhnert, Jens.
  • Schnieders R; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Peter SA; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Banijamali E; Department of Biology, Technical University of Darmstadt, Schnittspahnstr. 10, 64287, Darmstadt, Germany.
  • Riad M; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Biomedicum, Solnavägen 9, 17177, Stockholm, Sweden.
  • Altincekic N; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Biomedicum, Solnavägen 9, 17177, Stockholm, Sweden.
  • Bains JK; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Ceylan B; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Fürtig B; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Grün JT; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Hengesbach M; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Hohmann KF; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Hymon D; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Knezic B; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Oxenfarth A; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Petzold K; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Qureshi NS; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Richter C; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Schlagnitweit J; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Schlundt A; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Schwalbe H; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Stirnal E; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Sudakov A; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Vögele J; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Wacker A; Institute for Organic Chemistry and Chemical Biology, Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 7, 60438, Frankfurt/M., Germany.
  • Weigand JE; Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt/M., Germany.
  • Wirmer-Bartoschek J; Department of Medical Biochemistry and Biophysics, Karolinska Institute, Biomedicum, Solnavägen 9, 17177, Stockholm, Sweden.
  • Wöhnert J; EMBL Heidelberg, Meyerhofstraße 1, 69117, Heidelberg, Germany.
Biomol NMR Assign ; 15(1): 203-211, 2021 04.
Article in English | MEDLINE | ID: covidwho-1046684
Semantic information from SemMedBD (by NLM)
1. 5\' Untranslated Regions PART_OF 2019 novel coronavirus
Subject
5\' Untranslated Regions
Predicate
PART_OF
Object
2019 novel coronavirus
2. cis acting element PART_OF Betacoronavirus
Subject
cis acting element
Predicate
PART_OF
Object
Betacoronavirus
3. CISH AFFECTS RNA translation
Subject
CISH
Predicate
AFFECTS
Object
RNA translation
4. 5\' Untranslated Regions AFFECTS Virus Replication
Subject
5\' Untranslated Regions
Predicate
AFFECTS
Object
Virus Replication
5. Scientific Study USES Basis
Subject
Scientific Study
Predicate
USES
Object
Basis
6. 5' Untranslated Regions PART_OF 2019 novel coronavirus
Subject
5' Untranslated Regions
Predicate
PART_OF
Object
2019 novel coronavirus
7. cis acting element PART_OF Betacoronavirus
Subject
cis acting element
Predicate
PART_OF
Object
Betacoronavirus
8. CISH AFFECTS RNA translation
Subject
CISH
Predicate
AFFECTS
Object
RNA translation
9. 5' Untranslated Regions AFFECTS Virus Replication
Subject
5' Untranslated Regions
Predicate
AFFECTS
Object
Virus Replication
10. Scientific Study USES Basis
Subject
Scientific Study
Predicate
USES
Object
Basis
ABSTRACT
The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5'-untranslated region (5'-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5'-UUUCGU-3' hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive 1H, 13C and 15N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Magnetic Resonance Spectroscopy / 5' Untranslated Regions / Coronavirus Papain-Like Proteases / SARS-CoV-2 Language: English Journal: Biomol NMR Assign Journal subject: Molecular Biology / Nuclear Medicine Year: 2021 Document Type: Article Affiliation country: S12104-021-10007-w

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Magnetic Resonance Spectroscopy / 5' Untranslated Regions / Coronavirus Papain-Like Proteases / SARS-CoV-2 Language: English Journal: Biomol NMR Assign Journal subject: Molecular Biology / Nuclear Medicine Year: 2021 Document Type: Article Affiliation country: S12104-021-10007-w