Serological assays and host antibody detection in coronavirus-related disease diagnosis.
Arch Virol
; 166(3): 715-731, 2021 Mar.
Article
in English
| MEDLINE | ID: covidwho-1046770
ABSTRACT
Coronaviruses (CoV) are a family of viral pathogens that infect both birds and mammals, including humans. Seven human coronaviruses (HCoV) have been recognized so far. HCoV-229E, -OC43, -NL63, and -HKU1 account for one-third of common colds with mild symptoms. The other three members are severe acute respiratory syndrome (SARS)-CoV, Middle East respiratory syndrome (MERS)-CoV, and SARS-CoV-2. These viruses are responsible for SARS, MERS, and CoV disease 2019 (COVID-19), respectively. A variety of diagnostic techniques, including chest X-rays, computer tomography (CT) scans, analysis of viral nucleic acids, proteins, or whole virions, and host antibody detection using serological assays have been developed for the detection of these viruses. In this review, we discuss conventional serological tests, such as enzyme-linked immunosorbent assay (ELISA), western blot (WB), immunofluorescence assay (IFA), lateral flow immunoassay (LFIA), and chemiluminescence immunoassay (CLIA), as well as biosensor-based assays that have been developed for diagnosing HCoV-associated diseases since 2003, with an in-depth focus on COVID-19.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Severe Acute Respiratory Syndrome
/
Middle East Respiratory Syndrome Coronavirus
/
COVID-19 Serological Testing
/
SARS-CoV-2
/
COVID-19
/
Antibodies, Viral
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Arch Virol
Year:
2021
Document Type:
Article
Affiliation country:
S00705-020-04874-2
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