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Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA.
Eberle, Ute; Wimmer, Clara; Huber, Ingrid; Neubauer-Juric, Antonie; Valenza, Giuseppe; Ackermann, Nikolaus; Sing, Andreas.
  • Eberle U; Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Wimmer C; Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Huber I; Molecular Biology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Neubauer-Juric A; Unit of Veterinary Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Valenza G; Unit of Hospital Hygiene, Bavarian Health and Food Safety Authority, Erlangen, Germany.
  • Ackermann N; Unit of Virology, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.
  • Sing A; Public Health Microbiology Unit, Bavarian Health and Food Safety Authority, Oberschleißheim, Germany. Andreas.Sing@lgl.bayern.de.
Eur J Clin Microbiol Infect Dis ; 40(6): 1303-1308, 2021 Jun.
Article in English | MEDLINE | ID: covidwho-1053012
ABSTRACT
To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / COVID-19 Testing / COVID-19 Nucleic Acid Testing / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Qualitative research Limits: Humans Language: English Journal: Eur J Clin Microbiol Infect Dis Journal subject: Communicable Diseases / Microbiology Year: 2021 Document Type: Article Affiliation country: S10096-021-04159-9

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Full text: Available Collection: International databases Database: MEDLINE Main subject: RNA, Viral / COVID-19 Testing / COVID-19 Nucleic Acid Testing / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study / Qualitative research Limits: Humans Language: English Journal: Eur J Clin Microbiol Infect Dis Journal subject: Communicable Diseases / Microbiology Year: 2021 Document Type: Article Affiliation country: S10096-021-04159-9