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The m6A methylome of SARS-CoV-2 in host cells.
Liu, Jun'e; Xu, Yan-Peng; Li, Kai; Ye, Qing; Zhou, Hang-Yu; Sun, Hanxiao; Li, Xiaoyu; Yu, Liu; Deng, Yong-Qiang; Li, Rui-Ting; Cheng, Meng-Li; He, Bo; Zhou, Jia; Li, Xiao-Feng; Wu, Aiping; Yi, Chengqi; Qin, Cheng-Feng.
  • Liu J; State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
  • Xu YP; Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing, 100871, China.
  • Li K; Biomedical Pioneering Innovation Center, Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing, 100871, China.
  • Ye Q; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
  • Zhou HY; State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
  • Sun H; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
  • Li X; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
  • Yu L; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
  • Deng YQ; Suzhou Institute of System Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou, Jiangsu, 215000, China.
  • Li RT; State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
  • Cheng ML; State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
  • He B; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
  • Zhou J; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
  • Li XF; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
  • Wu A; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China.
  • Yi C; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, 100871, China.
  • Qin CF; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
Cell Res ; 31(4): 404-414, 2021 04.
Article in English | MEDLINE | ID: covidwho-1054016
ABSTRACT
The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a global health emergency because of its rapid spread and high mortality. The molecular mechanism of interaction between host and viral genomic RNA is yet unclear. We demonstrate herein that SARS-CoV-2 genomic RNA, as well as the negative-sense RNA, is dynamically N6-methyladenosine (m6A)-modified in human and monkey cells. Combined RIP-seq and miCLIP analyses identified a total of 8 m6A sites at single-base resolution in the genome. Especially, epidemic strains with mutations at these identified m6A sites have emerged worldwide, and formed a unique cluster in the US as indicated by phylogenetic analysis. Further functional experiments showed that m6A methylation negatively regulates SARS-CoV-2 infection. SARS-CoV-2 infection also triggered a global increase in host m6A methylome, exhibiting altered localization and motifs of m6A methylation in mRNAs. Altogether, our results identify m6A as a dynamic epitranscriptomic mark mediating the virus-host interaction.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Adenosine / Genome, Viral / SARS-CoV-2 Type of study: Randomized controlled trials Limits: Animals / Humans Language: English Journal: Cell Res Year: 2021 Document Type: Article Affiliation country: S41422-020-00465-7

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Adenosine / Genome, Viral / SARS-CoV-2 Type of study: Randomized controlled trials Limits: Animals / Humans Language: English Journal: Cell Res Year: 2021 Document Type: Article Affiliation country: S41422-020-00465-7