A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities.
Biotechniques
; 70(3): 149-159, 2021 03.
Article
in English
| MEDLINE | ID: covidwho-1054921
Preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
This scientific journal article is probably based on a previously available preprint. It has been identified through a machine matching algorithm, human confirmation is still pending.
See preprint
ABSTRACT
One goal of microbial ecology researchers is to capture the maximum amount of information from all organisms in a sample. The recent COVID-19 pandemic, caused by the RNA virus SARS-CoV-2, has highlighted a gap in traditional DNA-based protocols, including the high-throughput methods the authors previously established as field standards. To enable simultaneous SARS-CoV-2 and microbial community profiling, the authors compared the relative performance of two total nucleic acid extraction protocols with the authors' previously benchmarked protocol. The authors included a diverse panel of environmental and host-associated sample types, including body sites commonly swabbed for COVID-19 testing. Here the authors present results comparing the cost, processing time, DNA and RNA yield, microbial community composition, limit of detection and well-to-well contamination between these protocols.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
DNA, Viral
/
RNA, Ribosomal, 16S
/
High-Throughput Nucleotide Sequencing
/
Microbiota
/
SARS-CoV-2
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Biotechniques
Year:
2021
Document Type:
Article
Affiliation country:
Btn-2020-0153
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