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Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2.
Chang, Pai-Ling; Lin, Chia-Yi; Chen, Cheng-Pin; Lin, Yi-Chun; Hu, Hui-Chun; Cheng, Shu-Hsing; Cheng, Chien-Yu.
  • Chang PL; Department of Laboratory Medicine, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan. Electronic address: patty37021@gmail.com.
  • Lin CY; Department of Laboratory Medicine, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.
  • Chen CP; Department of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; School of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Lin YC; Department of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.
  • Hu HC; Department of Nursing, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.
  • Cheng SH; Department of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; School of Public Health, Taipei Medical University, Taipei, Taiwan.
  • Cheng CY; Department of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; School of Public Health, National Yang-Ming University, Taipei, Taiwan. Electronic address: s841060@gm.ym.edu.tw.
J Microbiol Immunol Infect ; 54(3): 522-526, 2021 Jun.
Article in English | MEDLINE | ID: covidwho-1056931
Semantic information from SemMedBD (by NLM)
1. Assay DIAGNOSES 2019 novel coronavirus
Subject
Assay
Predicate
DIAGNOSES
Object
2019 novel coronavirus
2. Assay DIAGNOSES 2019 novel coronavirus
Subject
Assay
Predicate
DIAGNOSES
Object
2019 novel coronavirus
ABSTRACT
To determine clinical performance of the single-target SARS-CoV-2 orf 1 ab reverse transcription-insulated isothermal PCR (RT-iiPCR) assay, the positive percentage agreement between this assay and a laboratory real-time RT-PCR assay was 96.8% (30 of 31; 95% confidence interval [CI], 90.5%-100%) and the negative percentage agreement was 97.1% (67 of 69; 95% CI, 93.1%-100%).
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Microbiol Immunol Infect Journal subject: Allergy and Immunology / Microbiology Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Nucleic Acid Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged / Young adult Language: English Journal: J Microbiol Immunol Infect Journal subject: Allergy and Immunology / Microbiology Year: 2021 Document Type: Article