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Self-Collected Saline Gargle Samples as an Alternative to Health Care Worker-Collected Nasopharyngeal Swabs for COVID-19 Diagnosis in Outpatients.
Goldfarb, David M; Tilley, Peter; Al-Rawahi, Ghada N; Srigley, Jocelyn A; Ford, Geoffrey; Pedersen, Heather; Pabbi, Abhilasha; Hannam-Clark, Stephanie; Charles, Marthe; Dittrick, Michelle; Gadkar, Vijay J; Pernica, Jeffrey M; Hoang, Linda M N.
  • Goldfarb DM; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada david.goldfarb@cw.bc.ca.
  • Tilley P; Division of Medical Microbiology, BC Children's Hospital & BC Women's Hospital & Health Centre, Vancouver, British Columbia, Canada.
  • Al-Rawahi GN; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Srigley JA; Division of Medical Microbiology, BC Children's Hospital & BC Women's Hospital & Health Centre, Vancouver, British Columbia, Canada.
  • Ford G; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Pedersen H; Division of Medical Microbiology, BC Children's Hospital & BC Women's Hospital & Health Centre, Vancouver, British Columbia, Canada.
  • Pabbi A; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
  • Hannam-Clark S; Division of Medical Microbiology, BC Children's Hospital & BC Women's Hospital & Health Centre, Vancouver, British Columbia, Canada.
  • Charles M; BC Centre for Disease Control, Vancouver, British Columbia, Canada.
  • Dittrick M; BC Centre for Disease Control, Vancouver, British Columbia, Canada.
  • Gadkar VJ; Clinical Informatics, BC Children's Hospital & BC Women's Hospital & Health Centre, Vancouver, British Columbia, Canada.
  • Pernica JM; Clinical Informatics, BC Children's Hospital & BC Women's Hospital & Health Centre, Vancouver, British Columbia, Canada.
  • Hoang LMN; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
J Clin Microbiol ; 59(4)2021 03 19.
Article in English | MEDLINE | ID: covidwho-1177520
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ABSTRACT
We assessed the performance, stability, and user acceptability of swab-independent self-collected saliva and saline mouth rinse/gargle sample types for the molecular detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in adults and school-aged children. Outpatients who had recently been diagnosed with COVID-19 or were presenting with suspected COVID-19 were asked to have a nasopharyngeal (NP) swab collected and provide at least one self-collected sample type. Participants were also asked about sample acceptability using a five-point Likert scale. For those previously diagnosed with COVID-19, all samples underwent real-time PCR testing using a lab-developed assay, and the majority were also tested using an FDA-authorized assay. For those presenting with suspected COVID-19, only those with a positive nasopharyngeal swab sample went on to have other samples tested. Saline mouth rinse/gargle and saliva samples were tested daily at time zero, day 1, and day 2 to assess nucleic acid stability at room temperature. Fifty participants (aged 4 to 71 years) were included; of these, 40 had at least one positive sample and were included in the primary sample yield analysis. Saline mouth rinse/gargle samples had a sensitivity of 98% (39/40), while saliva samples had a sensitivity of 79% (26/33). Both saline mouth rinse/gargle and saliva samples showed stable viral RNA detection after 2 days of room temperature storage. Mouth rinse/gargle samples had the highest (mean, 4.9) and health care worker (HCW)-collected NP swabs had the lowest acceptability scores (mean, 3.1). In conclusion, saline mouth rinse/gargle samples demonstrated higher combined user acceptability ratings and analytical performance than saliva and HCW-collected NP swabs. This sample type is a promising swab-independent option, particularly for outpatient self-collection in adults and school-aged children.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Outpatients / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Child / Humans Language: English Year: 2021 Document Type: Article Affiliation country: JCM.02427-20

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Outpatients / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Adult / Child / Humans Language: English Year: 2021 Document Type: Article Affiliation country: JCM.02427-20