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Isolation of SARS-CoV-2 strains carrying a nucleotide mutation, leading to a stop codon in the ORF 6 protein.
Delbue, Serena; D'Alessandro, Sarah; Signorini, Lucia; Dolci, Maria; Pariani, Elena; Bianchi, Michele; Fattori, Stefania; Modenese, Annalisa; Galli, Cristina; Eberini, Ivano; Ferrante, Pasquale.
  • Delbue S; Laboratory of Molecular Virology, Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy.
  • D'Alessandro S; Laboratory of Molecular Virology, Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy.
  • Signorini L; Laboratory of Molecular Virology, Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy.
  • Dolci M; Laboratory of Molecular Virology, Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy.
  • Pariani E; Department of Biomedical Science for Health, University of Milano, Milano, Italy.
  • Bianchi M; Department of Cardiology, Istituto Clinico Città Studi, Milano, Italy.
  • Fattori S; Interdepartmental Center of Diabetic Foot, Istituto Clinico Città Studi, Milano, Italy.
  • Modenese A; Laboratory of clinical analysis, Istituto Clinico Città Studi, Milano, Italy.
  • Galli C; Department of Biomedical Science for Health, University of Milano, Milano, Italy.
  • Eberini I; Department of Pharmacological and Biomolecular Sciences & DSRC, University of Milano, Milano, Italy.
  • Ferrante P; Laboratory of Molecular Virology, Department of Biomedical, Surgical and Dental Sciences, University of Milano, Milano, Italy.
Emerg Microbes Infect ; 10(1): 252-255, 2021 Dec.
Article in English | MEDLINE | ID: covidwho-1059032
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was isolated from the oro/pharyngeal swabs of two Italian COVID-19 patients, physicians in a COVID-19 division hospital, with different courses of the disease. The complete genome sequences show that the two isolates belong to the B1.1 lineage, but contain a nucleotide mutation in the ORF6, leading to a stop codon and to the deletion of 6 amino acids in the C terminus. This deletion was unique, compared to the currently available sequences deposited in the GISAID and GenBank database. It did not affect the in vitro viral replication, neither the neutralizing activities of the patients' antibodies. Based on homology analysis with other Coronaviruses, the two isolated lacked the ORF6 aminoacidic portion responsible for the inhibition of the antiviral Interferon (IFN)-based host response. IFN seems to have a dual role of in SARS-CoV-2 infected patients not only antiviral activity, but also a detrimental role in case of excessive production. A deletion in the SARS-CoV-2 ORF6 protein might have a specific, still unknown role in the viral pathogenesis.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Proteins / Point Mutation / Codon, Terminator / SARS-CoV-2 / COVID-19 Type of study: Case report / Diagnostic study Limits: Female / Humans / Male / Middle aged Language: English Journal: Emerg Microbes Infect Year: 2021 Document Type: Article Affiliation country: 22221751.2021.1884003

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Proteins / Point Mutation / Codon, Terminator / SARS-CoV-2 / COVID-19 Type of study: Case report / Diagnostic study Limits: Female / Humans / Male / Middle aged Language: English Journal: Emerg Microbes Infect Year: 2021 Document Type: Article Affiliation country: 22221751.2021.1884003