Neutralizing Antibody Responses to Severe Acute Respiratory Syndrome Coronavirus 2 in Coronavirus Disease 2019 Inpatients and Convalescent Patients.
Clin Infect Dis
; 71(10): 2688-2694, 2020 12 17.
Article
in English
| MEDLINE | ID: covidwho-1059702
ABSTRACT
BACKGROUND:
Coronavirus disease 2019 (COVID-19) is a pandemic with no specific antiviral treatments or vaccines. There is an urgent need for exploring the neutralizing antibodies from patients with different clinical characteristics.METHODS:
A total of 117 blood samples were collected from 70 COVID-19 inpatients and convalescent patients. Antibodies were determined with a modified cytopathogenic neutralization assay (NA) based on live severe acute respiratory syndrome coronavirus 2 and enzyme-linked immunosorbent assay (ELISA). The dynamics of neutralizing antibody levels at different time points with different clinical characteristics were analyzed.RESULTS:
The seropositivity rate reached up to 100.0% within 20 days since onset, and remained 100.0% till days 41-53. The total geometric mean titer was 1163.7 (95% confidence interval [CI], 128.5-208.6) by NA and 112 441.7 (95% CI, 9754.5-15 869.2) by ELISA. The antibody level by NA and ELISA peaked on days 31-40 since onset, and then decreased slightly. In multivariate generalized estimating equation analysis, patients aged 31-45, 46-60, and 61-84 years had a higher neutralizing antibody level than those aged 16-30 years (ß = 1.0470, Pâ =â .0125; ß = 1.0613, Pâ =â .0307; ß = 1.3713, Pâ =â .0020). Patients with a worse clinical classification had a higher neutralizing antibody titer (ßâ =â 0.4639, Pâ =â .0227).CONCLUSIONS:
The neutralizing antibodies were detected even at the early stage of disease, and a significant response was shown in convalescent patients.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Antibodies, Neutralizing
/
COVID-19
Type of study:
Prognostic study
Topics:
Vaccines
Limits:
Adolescent
/
Adult
/
Humans
/
Middle aged
/
Young adult
Language:
English
Journal:
Clin Infect Dis
Journal subject:
Communicable Diseases
Year:
2020
Document Type:
Article
Affiliation country:
Cid
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