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Analysis of viral load in different specimen types and serum antibody levels of COVID-19 patients.
Li, Ling; Tan, Chianru; Zeng, Jia; Luo, Chen; Hu, Shi; Peng, Yanke; Li, Wenjuan; Xie, Zhixiong; Ling, Yueming; Zhang, Xuejun; Deng, E; Xu, Haixia; Wang, Jue; Xie, Yudi; Zhou, Yaling; Zhang, Wei; Guo, Yong; Liu, Zhong.
  • Li L; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, People's Republic of China.
  • Tan C; Key Laboratory of Transfusion Adverse Reactions, Chinese Academy of Medical Sciences, Chengdu, 610052, Sichuan, People's Republic of China.
  • Zeng J; Department of Biomedical Engineering, School of Medicine, Tsinghua University, 30 Shuangqing Road, Beijing, 100084, People's Republic of China.
  • Luo C; Department of Aviation Disease, Naval Medical Center of PLA, Second Military Medical University, Shanghai, 200052, People's Republic of China.
  • Hu S; The Maternal and Child Health Hospital of Hubei Province, Guanggu District, Wuhan, 430070, Hubei, People's Republic of China.
  • Peng Y; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, People's Republic of China.
  • Li W; Key Laboratory of Transfusion Adverse Reactions, Chinese Academy of Medical Sciences, Chengdu, 610052, Sichuan, People's Republic of China.
  • Xie Z; Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, 200433, People's Republic of China.
  • Ling Y; Department of Biomedical Engineering, School of Medicine, Tsinghua University, 30 Shuangqing Road, Beijing, 100084, People's Republic of China.
  • Zhang X; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, People's Republic of China.
  • Deng E; Anhui Medical University, Hefei, 230032, People's Republic of China.
  • Xu H; The Maternal and Child Health Hospital of Hubei Province, Guanggu District, Wuhan, 430070, Hubei, People's Republic of China.
  • Wang J; Department of Clinical Laboratory Science of NO. 909 Hospital of PLA Joint Support Force, Zhangzhou, 363000, People's Republic of China.
  • Xie Y; The Maternal and Child Health Hospital of Hubei Province, Guanggu District, Wuhan, 430070, Hubei, People's Republic of China.
  • Zhou Y; Department of Clinical Laboratory Science of NO. 910 Hospital of PLA Joint Support Force, Quanzhou, 362000, People's Republic of China.
  • Zhang W; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, People's Republic of China.
  • Guo Y; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, People's Republic of China.
  • Liu Z; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, Sichuan, People's Republic of China.
J Transl Med ; 19(1): 30, 2021 01 07.
Article in English | MEDLINE | ID: covidwho-1059718
ABSTRACT

BACKGROUND:

COVID-19 has caused a global pandemic and the death toll is increasing. However, there is no definitive information regarding the type of clinical specimens that is the best for SARS-CoV-2 detection, the antibody levels in patients with different duration of disease, and the relationship between antibody level and viral load.

METHODS:

Nasopharyngeal swabs, anal swabs, saliva, blood, and urine specimens were collected from patients with a course of disease ranging from 7 to 69 days. Viral load in different specimen types was measured using droplet digital PCR (ddPCR). Meanwhile, anti-nucleocapsid protein (anti-N) IgM and IgG antibodies and anti-spike protein receptor-binding domain (anti-S-RBD) IgG antibody in all serum samples were tested using ELISA.

RESULTS:

The positive detection rate in nasopharyngeal swab was the highest (54.05%), followed by anal swab (24.32%), and the positive detection rate in saliva, blood, and urine was 16.22%, 10.81%, and 5.41%, respectively. However, some patients with negative nasopharyngeal swabs had other specimens tested positive. There was no significant correlation between antibody level and days after symptoms onset or viral load.

CONCLUSIONS:

Other specimens could be positive in patients with negative nasopharyngeal swabs, suggesting that for patients in the recovery period, specimens other than nasopharyngeal swabs should also be tested to avoid false negative results, and anal swabs are recommended. The antibody level had no correlation with days after symptoms onset or the viral load of nasopharyngeal swabs, suggesting that the antibody level may also be affected by other factors.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Load / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Country/Region as subject: Asia Language: English Journal: J Transl Med Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Viral Load / SARS-CoV-2 / COVID-19 / Antibodies, Viral Type of study: Diagnostic study / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Country/Region as subject: Asia Language: English Journal: J Transl Med Year: 2021 Document Type: Article