Assessment of hepatoprotective, renal protective and anti-genotoxic effect of novel synthesized complexes of Ca(II), Mg(II) and Cr(III) with 2,6-dichloroindophenol against Acrylamide toxicity in male rats

ABSTRACT

Herein this study aimed to prepare and spectroscopically investigate magnesium(II), calcium(II) and chromium(III) complexes with 2,6-dichloroindo phenol sodium hydrate (Dich). The suggestion formula can be presented as [Mg(Dich)2(H2O)4].5H2O, [Ca(Dich)2(H2O)4].2H2O and [Cr(Dich)3(H2O)3].3H2O according to micro analytical, conductivity, FTIR, magnetic susceptibility, UV-Visible, TG-DrTGA, SEM and TEM studies. The analyses revealed that the Dich ligand acts as a monodentate chelating through the oxygen atom of-OH phenolic group with an octahedral geometry around the central metal ions. The six-coordination sphere was completing by coordinated water molecules. This study aimed to evaluate the potent antioxidant, antihepatorenal toxicity and antigenotoxic effects of acrylamide (AC) and role of the novel complexes Ca/Dich, Mg/Dich and Cr/Dich in alleviating hepatorenaltoxicity, oxidative injury and genotoxicity induced by AC in male albino rats. After 30 consecutive days of exposure, some biochemical parameters were measured as hepatic aminotransferases enzymes, lipid profile, kidney function parameters, TNF-α, IL-6, markers of inflammation, tissue damage LDH, CRP, mitochondrial potential activities, MPO, XO, CRP,SDH , MMP , MDA levels and antioxidant enzymes (SOD , CAT , GRx and GST) ,ATP content with histological, TEM examination of the hepatic and renal tissues and Alkaline comet assay in hepatic tissues . AC induced biochemical and cellular alterations in the hepatic and renal tissues and theses toxicity were alleviated greatly after treatment of male rats with the novel complexes Ca/Dich, Mg/Dich and Cr/Dich which afforded protection against hepatorenal injury and oxidative stress resulting from AC toxicity. Consequently, the novel complexes improved thehepatic enzymes and improved the antioxidant capacities in the hepatic and renal tissues. © 2021 World Research Association. All rights reserved.