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Decreased GLUT1/NHE1 RNA expression in whole blood predicts disease severity in patients with COVID-19.
Mustroph, Julian; Hupf, Julian; Hanses, Frank; Evert, Katja; Baier, Maria J; Evert, Matthias; Meindl, Christine; Wagner, Stefan; Hubauer, Ute; Pietrzyk, Gabriela; Leininger, Simon; Staudner, Stephan; Vogel, Manuel; Wallner, Stefan; Zimmermann, Markus; Sossalla, Samuel; Maier, Lars S; Jungbauer, Carsten.
  • Mustroph J; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Hupf J; Emergency Department, University Hospital Regensburg, Regensburg, Germany.
  • Hanses F; Emergency Department, University Hospital Regensburg, Regensburg, Germany.
  • Evert K; Department of Infection Prevention and Infectious Diseases, University Hospital Regensburg, Regensburg, Germany.
  • Baier MJ; Institute of Pathology, University Hospital Regensburg, Regensburg, Germany.
  • Evert M; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Meindl C; Institute of Pathology, University Hospital Regensburg, Regensburg, Germany.
  • Wagner S; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Hubauer U; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Pietrzyk G; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Leininger S; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Staudner S; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Vogel M; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Wallner S; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Zimmermann M; Department of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany.
  • Sossalla S; Emergency Department, University Hospital Regensburg, Regensburg, Germany.
  • Maier LS; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
  • Jungbauer C; Department of Internal Medicine II (Cardiology), University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, Regensburg, 93053, Germany.
ESC Heart Fail ; 8(1): 309-316, 2021 02.
Article in English | MEDLINE | ID: covidwho-1064347
ABSTRACT

AIMS:

We aimed to assess whether expression of whole-blood RNA of sodium proton exchanger 1 (NHE1) and glucose transporter 1 (GLUT1) is associated with COVID-19 infection and outcome in patients presenting to the emergency department with respiratory infections. Furthermore, we investigated NHE1 and GLUT1 expression in the myocardium of deceased COVID-19 patients. METHODS AND

RESULTS:

Whole-blood quantitative assessment of NHE1 and GLUT1 RNA was performed using quantitative PCR in patients with respiratory infection upon first contact in the emergency department and subsequently stratified by SARS-CoV-2 infection status. Assessment of NHE1 and GLUT1 RNA using PCR was also performed in left ventricular myocardium of deceased COVID-19 patients. NHE1 expression is up-regulated in whole blood of patients with COVID-19 compared with other respiratory infections at first medical contact in the emergency department (control 0.0021 ± 0.0002, COVID-19 0.0031 ± 0.0003, P = 0.01). The ratio of GLUT1 to NHE1 is significantly decreased in the blood of COVID-19 patients who are subsequently intubated and/or die (severe disease) compared with patients with moderate disease (moderate disease 0.497 ± 0.083 vs. severe disease 0.294 ± 0.0336, P = 0.036). This ratio is even further decreased in the myocardium of patients who deceased from COVID-19 in comparison with the myocardium of non-infected donors.

CONCLUSIONS:

NHE1 and GLUT1 may be critically involved in the disease progression of SARS-CoV-2 infection. We show here that SARS-CoV-2 infection critically disturbs ion channel expression in the heart. A decreased ratio of GLUT1/NHE1 could potentially serve as a biomarker for disease severity in patients with COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Glucose Transporter Type 1 / Sodium-Hydrogen Exchanger 1 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Female / Humans / Male / Middle aged Language: English Journal: ESC Heart Fail Year: 2021 Document Type: Article Affiliation country: Ehf2.13063

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Glucose Transporter Type 1 / Sodium-Hydrogen Exchanger 1 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Female / Humans / Male / Middle aged Language: English Journal: ESC Heart Fail Year: 2021 Document Type: Article Affiliation country: Ehf2.13063