Intrauterine growth restriction affects diaphragm function in adult female and male mice.
Pediatr Pulmonol
; 55(1): 229-235, 2020 01.
Article
in English
| MEDLINE | ID: covidwho-1064409
ABSTRACT
BACKGROUND:
In utero diaphragm development is critically important for postnatal respiratory function and any disturbance to fetal development may lead to diaphragm dysfunction and respiratory complications in the postnatal period. Intrauterine growth restriction (IUGR) has been shown to affect respiratory function in a sex-dependent manner; however, the effect of IUGR on diaphragm function is unknown.AIM:
This study used a maternal hypoxia-induced mouse model of IUGR to investigate the impact of IUGR on diaphragm function and structure in male and female adult offspring. MATERIALS ANDMETHODS:
Pregnant BALB/c mice were housed under hypoxic conditions (10.5% O2 ) from gestational days 11 to 17.5 and then returned to normoxic conditions. Control mice were housed under normoxic conditions throughout pregnancy. At 8 weeks of age, offspring were euthanized and diaphragms isolated for functional assessment in organ bath experiments and for histological analysis.RESULTS:
IUGR offspring were lighter at birth and remained lighter at 8 weeks of age compared to Controls. While diaphragm force (maximal or twitch) was not affected by treatment or sex, the IUGR group exhibited a longer half-relaxation time after twitch contractions compared to Control. Female offspring had a lower maximum rate of force development and higher fatigue resistance compared to males, independent of IUGR. There was no difference in the diaphragm myofibre cross-sectional area between groups or sexes.CONCLUSION:
Sex and IUGR independently affect diaphragm contraction in adult mice without changes in structure. This study demonstrates that IUGR affects diaphragm contractile function in later life and could impair respiratory function if exacerbated under conditions of increased respiratory load.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Diaphragm
/
Fetal Growth Retardation
Type of study:
Experimental Studies
/
Observational study
/
Randomized controlled trials
Topics:
Long Covid
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Pediatr Pulmonol
Journal subject:
Pediatrics
Year:
2020
Document Type:
Article
Affiliation country:
Ppul.24519
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