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Experimental design approach for development of spectrofluorimetric method for determination of favipiravir; a potential therapeutic agent against COVID-19 virus: Application to spiked human plasma.
Megahed, Safa M; Habib, Ahmed A; Hammad, Sherin F; Kamal, Amira H.
  • Megahed SM; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt. Electronic address: safa.megahed@pharm.tanta.edu.eg.
  • Habib AA; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
  • Hammad SF; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
  • Kamal AH; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc ; 249: 119241, 2021 Mar 15.
Article in English | MEDLINE | ID: covidwho-1065570
Semantic information from SemMedBD (by NLM)
1. favipiravir COEXISTS_WITH BORATE BUFFER
Subject
favipiravir
Predicate
COEXISTS_WITH
Object
BORATE BUFFER
2. favipiravir COEXISTS_WITH human plasma
Subject
favipiravir
Predicate
COEXISTS_WITH
Object
human plasma
3. favipiravir COEXISTS_WITH BORATE BUFFER
Subject
favipiravir
Predicate
COEXISTS_WITH
Object
BORATE BUFFER
4. favipiravir COEXISTS_WITH human plasma
Subject
favipiravir
Predicate
COEXISTS_WITH
Object
human plasma
ABSTRACT
The present work describes development of rapid, robust, sensitive and green spectrofluorimetric method for determination of favipiravir (FAV). Different factors affecting fluorescence were carefully studied and Box Behnken Design was applied to optimize experimental parameters. The proposed method is based on measuring native fluorescence of FAV in 0.2 M borate buffer (pH 8.0) at 432 nm after excitation at 361 nm. There was a linear relationship between FAV concentration and relative fluorescence intensity over the range 40-280 ng/mL with limit of detection of 9.44 ng/mL and quantitation limit of 28.60 ng/mL. The method was successfully implemented for determination of FAV in its pharmaceutical formulation with mean % recovery of 99.26 ± 0.87. Moreover, the high sensitivity of the method allowed determination of FAV in spiked human plasma over a range of 48-192 ng/mL. The proposed spectrofluorimetric method was proved to be eco-friendly according to analytical eco-scale.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Pyrazines / Spectrometry, Fluorescence / Amides / COVID-19 Type of study: Diagnostic study / Evaluation study Limits: Humans Language: English Journal: Spectrochim Acta A Mol Biomol Spectrosc Journal subject: Molecular Biology Year: 2021 Document Type: Article

Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Pyrazines / Spectrometry, Fluorescence / Amides / COVID-19 Type of study: Diagnostic study / Evaluation study Limits: Humans Language: English Journal: Spectrochim Acta A Mol Biomol Spectrosc Journal subject: Molecular Biology Year: 2021 Document Type: Article