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A stable platform for the production of virus-like particles pseudotyped with the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein.
Roy, Sylvie; Ghani, Karim; de Campos-Lima, Pedro O; Caruso, Manuel.
  • Roy S; CHU de Québec-Université Laval Research Center (Oncology division), Université Laval Cancer Research Center, Québec, Qc, Canada.
  • Ghani K; CHU de Québec-Université Laval Research Center (Oncology division), Université Laval Cancer Research Center, Québec, Qc, Canada; BioVec Pharma, Québec, Qc, Canada.
  • de Campos-Lima PO; Boldrini Children's Center, Campinas, Brazil; Functional and Molecular Biology Graduate Program, State University of Campinas, Brazil.
  • Caruso M; CHU de Québec-Université Laval Research Center (Oncology division), Université Laval Cancer Research Center, Québec, Qc, Canada; BioVec Pharma, Québec, Qc, Canada; Department of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Université Laval, Québec, Qc, Canada. Electron
Virus Res ; 295: 198305, 2021 04 02.
Article in English | MEDLINE | ID: covidwho-1065651
ABSTRACT
In this study, we showed that a codon optimized version of the spike (S) protein of SARS-CoV-2 can migrate to the cell membrane. However, efficient production of Moloney murine leukemia (MLV) infectious viral particles was only achieved with stable expression of a shorter S version in C-terminal (ΔS) in MLV Gag-pol expressing cells. As compared to transient transfections, this platform generated viruses with a 1000-fold higher titer. ΔS was 15-times more efficiently incorporated into VLPs as compared to S, and that was not due to steric interference between the cytoplasmic tail and the MLV capsid, as similar differences were also observed with extracellular vesicles. The amount of ΔS incorporated into VLPs released from producer cells was high and estimated at 1.25 µg/mL S2 equivalent (S is comprised of S1 and S2). The resulting VLPs could potentially be used alone or as a boost of other immunization strategies for COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virion / Spike Glycoprotein, Coronavirus / COVID-19 Vaccines Topics: Vaccines Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2021 Document Type: Article Affiliation country: J.virusres.2021.198305

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virion / Spike Glycoprotein, Coronavirus / COVID-19 Vaccines Topics: Vaccines Limits: Humans Language: English Journal: Virus Res Journal subject: Virology Year: 2021 Document Type: Article Affiliation country: J.virusres.2021.198305