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Detection of SARS-CoV-2 Viral Particles Using Direct, Reagent-Free Electrochemical Sensing.
Yousefi, Hanie; Mahmud, Alam; Chang, Dingran; Das, Jagotamoy; Gomis, Surath; Chen, Jenise B; Wang, Hansen; Been, Terek; Yip, Lily; Coomes, Eric; Li, Zhijie; Mubareka, Samira; McGeer, Allison; Christie, Natasha; Gray-Owen, Scott; Cochrane, Alan; Rini, James M; Sargent, Edward H; Kelley, Shana O.
  • Yousefi H; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada.
  • Mahmud A; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON M5S 3G4, Canada.
  • Chang D; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada.
  • Das J; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada.
  • Gomis S; The Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON M5S 3G4, Canada.
  • Chen JB; Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.
  • Wang H; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada.
  • Been T; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Yip L; Sunnybrook Research Institute, Toronto, ON M4N 3N5, Canada.
  • Coomes E; Division of Infectious Disease, Department of Medicine, University of Toronto, Toronto, ON M5G 2C4, Canada.
  • Li Z; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Mubareka S; Sunnybrook Research Institute, Toronto, ON M4N 3N5, Canada.
  • McGeer A; Department of Microbiology, Sinai Health System, Toronto, ON M5G 1X5, Canada.
  • Christie N; Combined Containment Level 3 Unit, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Gray-Owen S; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Cochrane A; Combined Containment Level 3 Unit, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Rini JM; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Sargent EH; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Kelley SO; Department of Biochemistry, University of Toronto, Toronto, ON M5G 1M1, Canada.
J Am Chem Soc ; 143(4): 1722-1727, 2021 02 03.
Article in English | MEDLINE | ID: covidwho-1065802
ABSTRACT
The development of new methods for direct viral detection using streamlined and ideally reagent-free assays is a timely and important, but challenging, problem. The challenge of combatting the COVID-19 pandemic has been exacerbated by the lack of rapid and effective methods to identify viral pathogens like SARS-CoV-2 on-demand. Existing gold standard nucleic acid-based approaches require enzymatic amplification to achieve clinically relevant levels of sensitivity and are not typically used outside of a laboratory setting. Here, we report reagent-free viral sensing that directly reads out the presence of viral particles in 5 minutes using only a sensor-modified electrode chip. The approach relies on a class of electrode-tethered sensors bearing an analyte-binding antibody displayed on a negatively charged DNA linker that also features a tethered redox probe. When a positive potential is applied, the sensor is transported to the electrode surface. Using chronoamperometry, the presence of viral particles and proteins can be detected as these species increase the hydrodynamic drag on the sensor. This report is the first virus-detecting assay that uses the kinetic response of a probe/virus complex to analyze the complexation state of the antibody. We demonstrate the performance of this sensing approach as a means to detect, within 5 min, the presence of the SARS-CoV-2 virus and its associated spike protein in test samples and in unprocessed patient saliva.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virion / Biosensing Techniques / Electrochemical Techniques / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: J Am Chem Soc Year: 2021 Document Type: Article Affiliation country: Jacs.0c10810

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virion / Biosensing Techniques / Electrochemical Techniques / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Prognostic study Limits: Humans Language: English Journal: J Am Chem Soc Year: 2021 Document Type: Article Affiliation country: Jacs.0c10810