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Rapid electrochemical detection of coronavirus SARS-CoV-2.
Chaibun, Thanyarat; Puenpa, Jiratchaya; Ngamdee, Tatchanun; Boonapatcharoen, Nimaradee; Athamanolap, Pornpat; O'Mullane, Anthony Peter; Vongpunsawad, Sompong; Poovorawan, Yong; Lee, Su Yin; Lertanantawong, Benchaporn.
  • Chaibun T; Biosensors Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom, Thailand.
  • Puenpa J; Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Ngamdee T; Department of Biotechnology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
  • Boonapatcharoen N; Pilot Plant Development and Training Institute (PDTI), King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
  • Athamanolap P; Biosensors Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Mahidol University, Nakhon Pathom, Thailand.
  • O'Mullane AP; School of Chemistry and Physics, Queensland University of Technology (QUT), Brisbane, QLD, Australia.
  • Vongpunsawad S; Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Poovorawan Y; Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
  • Lee SY; Faculty of Applied Sciences, AIMST University, Bedong, Kedah, Malaysia.
  • Lertanantawong B; Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio), AIMST University, Bedong, Kedah, Malaysia.
Nat Commun ; 12(1): 802, 2021 02 05.
Article in English | MEDLINE | ID: covidwho-1065865
ABSTRACT
Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/µL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Electrochemical Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2021 Document Type: Article Affiliation country: S41467-021-21121-7

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Biosensing Techniques / Electrochemical Techniques / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Prognostic study Limits: Humans Language: English Journal: Nat Commun Journal subject: Biology / Science Year: 2021 Document Type: Article Affiliation country: S41467-021-21121-7