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Altered high-density lipoprotein composition and functions during severe COVID-19.
Begue, Floran; Tanaka, Sébastien; Mouktadi, Zarouki; Rondeau, Philippe; Veeren, Bryan; Diotel, Nicolas; Tran-Dinh, Alexy; Robert, Tiphaine; Vélia, Erick; Mavingui, Patrick; Lagrange-Xélot, Marie; Montravers, Philippe; Couret, David; Meilhac, Olivier.
  • Begue F; INSERM, UMR 1188 Diabète atherothombose Réunion Océan Indien (DéTROI), Université de La Réunion, Saint-Denis de La Réunion, France.
  • Tanaka S; INSERM, UMR 1188 Diabète atherothombose Réunion Océan Indien (DéTROI), Université de La Réunion, Saint-Denis de La Réunion, France.
  • Mouktadi Z; AP-HP, Service d'Anesthésie-Réanimation, CHU Bichat-Claude Bernard, 75018, Paris, France.
  • Rondeau P; INSERM, UMR 1188 Diabète atherothombose Réunion Océan Indien (DéTROI), Université de La Réunion, Saint-Denis de La Réunion, France.
  • Veeren B; INSERM, UMR 1188 Diabète atherothombose Réunion Océan Indien (DéTROI), Université de La Réunion, Saint-Denis de La Réunion, France.
  • Diotel N; INSERM, UMR 1188 Diabète atherothombose Réunion Océan Indien (DéTROI), Université de La Réunion, Saint-Denis de La Réunion, France.
  • Tran-Dinh A; INSERM, UMR 1188 Diabète atherothombose Réunion Océan Indien (DéTROI), Université de La Réunion, Saint-Denis de La Réunion, France.
  • Robert T; AP-HP, Service d'Anesthésie-Réanimation, CHU Bichat-Claude Bernard, 75018, Paris, France.
  • Vélia E; Université de Paris, UFR Denis Diderot, Paris, France.
  • Mavingui P; AP-HP, Service de Biochimie, CHU Bichat-Claude Bernard, 75018, Paris, France.
  • Lagrange-Xélot M; Clinique Sainte-Clotilde, Groupe Clinifutur, Pôle mère enfant, 97490, Sainte-Clotilde, La Réunion, France.
  • Montravers P; UMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), INSERM 1187, CNRS 9192, IRD 249, Université de La Réunion, 2 rue Maxime Rivière (GIP CYROI), 97490, Sainte-Clotilde, La Réunion, France.
  • Couret D; Service des maladies infectieuses, CHU de La Réunion, Saint-Denis, France.
  • Meilhac O; AP-HP, Service d'Anesthésie-Réanimation, CHU Bichat-Claude Bernard, 75018, Paris, France.
Sci Rep ; 11(1): 2291, 2021 01 27.
Article in English | MEDLINE | ID: covidwho-1065945
ABSTRACT
Coronavirus disease 2019 (COVID-19) pandemic is affecting millions of patients worldwide. The consequences of initial exposure to SARS-CoV-2 go beyond pulmonary damage, with a particular impact on lipid metabolism. Decreased levels in HDL-C were reported in COVID-19 patients. Since HDL particles display antioxidant, anti-inflammatory and potential anti-infectious properties, we aimed at characterizing HDL proteome and functionality during COVID-19 relative to healthy subjects. HDLs were isolated from plasma of 8 severe COVID-19 patients sampled at admission to intensive care unit (Day 1, D1) at D3 and D7, and from 16 sex- and age-matched healthy subjects. Proteomic analysis was performed by LC-MS/MS. The relative amounts of proteins identified in HDLs were compared between COVID-19 and controls. apolipoprotein A-I and paraoxonase 1 were confirmed by Western-blot analysis to be less abundant in COVID-19 versus controls, whereas serum amyloid A and alpha-1 antitrypsin were higher. HDLs from patients were less protective in endothelial cells stiumalted by TNFα (permeability, VE-cadherin disorganization and apoptosis). In these conditions, HDL inhibition of apoptosis was blunted in COVID-19 relative to controls. In conclusion, we show major changes in HDL proteome and decreased functionality in severe COVID-19 patients.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Lipoproteins, HDL Type of study: Observational study Limits: Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-81638-1

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 / Lipoproteins, HDL Type of study: Observational study Limits: Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-81638-1