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Use of exhaled breath condensate (EBC) in the diagnosis of SARS-COV-2 (COVID-19).
Ryan, Daniel J; Toomey, Sinead; Madden, Stephen F; Casey, Michelle; Breathnach, Oscar S; Morris, Patrick G; Grogan, Liam; Branagan, Peter; Costello, Richard W; De Barra, Eoghan; Hurley, Killian; Gunaratnam, Cedric; McElvaney, Noel G; OBrien, Michael Emmet; Sulaiman, Imran; Morgan, Ross K; Hennessy, Bryan T.
  • Ryan DJ; Department of Molecular Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland danennis100@gmail.com.
  • Toomey S; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • Madden SF; Department of Molecular Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland.
  • Casey M; Data Science Centre, Royal College of Surgeons in Ireland, Dublin, Ireland.
  • Breathnach OS; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • Morris PG; Department of Oncology, Beaumont Hospital, Dublin, Ireland.
  • Grogan L; Department of Oncology, Beaumont Hospital, Dublin, Ireland.
  • Branagan P; Department of Oncology, Beaumont Hospital, Dublin, Ireland.
  • Costello RW; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • De Barra E; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • Hurley K; Department of International Health and Tropical Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland.
  • Gunaratnam C; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • McElvaney NG; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • OBrien ME; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • Sulaiman I; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • Morgan RK; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
  • Hennessy BT; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland.
Thorax ; 76(1): 86-88, 2021 01.
Article in English | MEDLINE | ID: covidwho-1066942
ABSTRACT
False negatives from nasopharyngeal swabs (NPS) using reverse transcriptase PCR (RT-PCR) in SARS-CoV-2 are high. Exhaled breath condensate (EBC) contains lower respiratory droplets that may improve detection. We performed EBC RT-PCR for SARS-CoV-2 genes (E, S, N, ORF1ab) on NPS-positive (n=16) and NPS-negative/clinically positive COVID-19 patients (n=15) using two commercial assays. EBC detected SARS-CoV-2 in 93.5% (29/31) using the four genes. Pre-SARS-CoV-2 era controls (n=14) were negative. EBC was positive in NPS negative/clinically positive patients in 66.6% (10/15) using the identical E and S (E/S) gene assay used for NPS, 73.3% (11/15) using the N/ORF1ab assay and 14/15 (93.3%) combined.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Breath Tests / RNA, Viral / Exhalation / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Thorax Year: 2021 Document Type: Article Affiliation country: Thoraxjnl-2020-215705

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Breath Tests / RNA, Viral / Exhalation / COVID-19 Testing / SARS-CoV-2 / COVID-19 Type of study: Cohort study / Diagnostic study / Observational study / Prognostic study Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: English Journal: Thorax Year: 2021 Document Type: Article Affiliation country: Thoraxjnl-2020-215705