Mediators of SARS-CoV-2 entry are preferentially enriched in cardiomyocytes.
Hereditas
; 158(1): 4, 2021 Jan 04.
Article
in English
| MEDLINE | ID: covidwho-1067345
ABSTRACT
BACKGROUND:
The coronavirus disease 2019 (COVID-19) has spread rapidly around the world. In addition to common respiratory symptoms such as cough and fever, some patients also have cardiac injury, however, the mechanism of cardiac injury is not clear. In this study, we analyzed the RNA expression atlases of angiotensin-converting enzyme 2(ACE2), cathepsin B (CTSB) and cathepsin L (CTSL) in the human embryonic heart at single-cell resolution.RESULTS:
The results showed that ACE2 was preferentially enriched in cardiomyocytes. Interestingly, serine protease transmembrane serine protease 2 (TMPRSS2) had less expression in cardiomyocytes, but CTSB and CTSL, which belonged to cell protease, could be found to be enriched in cardiomyocytes. The results of enrichment analysis showed that differentially expressed genes (DEGs) in ACE2-positive cardiomyocytes were mainly enriched in the processes of cardiac muscle contraction, regulation of cardiac conduction, mitochondrial respiratory chain, ion channel binding, adrenergic signaling in cardiomyocytes and viral transcription.CONCLUSIONS:
Our study suggests that both atrial and ventricular cardiomyocytes are potentially susceptible to severe acute respiratory syndrome coronavirus-2(SARS-CoV-2), and SARS-CoV-2 may enter ventricular cardiomyocytes using CTSB/CTSL for S protein priming. This may be the partial cellular mechanism of cardiac injury in patients with COVID-19.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Gene Expression Regulation, Developmental
/
Myocytes, Cardiac
/
Single-Cell Analysis
/
SARS-CoV-2
/
COVID-19
/
Heart
Type of study:
Observational study
Limits:
Humans
Language:
English
Journal:
Hereditas
Year:
2021
Document Type:
Article
Affiliation country:
S41065-020-00168-4
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