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PCR past, present and future.
Zhu, Hanliang; Zhang, Haoqing; Xu, Ying; Lassáková, Sona; Korabecná, Marie; Neuzil, Pavel.
  • Zhu H; Department of Microsystem Engineering, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, PR China.
  • Zhang H; Department of Microsystem Engineering, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, PR China.
  • Xu Y; Department of Microsystem Engineering, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, PR China.
  • Lassáková S; Institute of Biology & Medical Genetics, First Faculty of Medicine, Charles University & General University Hospital in Prague, Purkyne Institute, Albertov 4, Praha 2 128 00, Czech Republic.
  • Korabecná M; Institute of Biology & Medical Genetics, First Faculty of Medicine, Charles University & General University Hospital in Prague, Purkyne Institute, Albertov 4, Praha 2 128 00, Czech Republic.
  • Neuzil P; Department of Microsystem Engineering, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, PR China.
Biotechniques ; 69(4): 317-325, 2020 10.
Article in English | MEDLINE | ID: covidwho-1067502
ABSTRACT
PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Polymerase Chain Reaction Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Language: English Journal: Biotechniques Year: 2020 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Polymerase Chain Reaction Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Language: English Journal: Biotechniques Year: 2020 Document Type: Article