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Susceptibility of neuroblastoma and glioblastoma cell lines to SARS-CoV-2 infection.
Bielarz, Valéry; Willemart, Kévin; Avalosse, Noémie; De Swert, Kathleen; Lotfi, Riselane; Lejeune, Noémie; Poulain, Florian; Ninanne, Noelle; Gilloteaux, Jacques; Gillet, Nicolas; Nicaise, Charles.
  • Bielarz V; URPhyM, NARILIS, Université de Namur, Namur, Belgium. Electronic address: valery.bielarz@unamur.be.
  • Willemart K; URVI, Université de Namur, Namur, Belgium. Electronic address: kevin.willemart@unamur.be.
  • Avalosse N; URPhyM, NARILIS, Université de Namur, Namur, Belgium. Electronic address: noemie.avalosse@student.unamur.be.
  • De Swert K; URPhyM, NARILIS, Université de Namur, Namur, Belgium. Electronic address: kathleen.deswert@unamur.be.
  • Lotfi R; URPhyM, NARILIS, Université de Namur, Namur, Belgium. Electronic address: riselane.lotfi@student.unamur.be.
  • Lejeune N; URVI, Université de Namur, Namur, Belgium. Electronic address: noemie.lejeune@unamur.be.
  • Poulain F; URVI, Université de Namur, Namur, Belgium. Electronic address: florian.poulain@unamur.be.
  • Ninanne N; URBC, Université de Namur, Namur, Belgium. Electronic address: noelle.ninanne@unamur.be.
  • Gilloteaux J; URPhyM, NARILIS, Université de Namur, Namur, Belgium; Department of Anatomical Sciences, St George's University School of Medicine, Newcastle upon Tyne, United Kingdom. Electronic address: JGilloteaux@sgu.edu.
  • Gillet N; URVI, Université de Namur, Namur, Belgium. Electronic address: nicolas.gillet@unamur.be.
  • Nicaise C; URPhyM, NARILIS, Université de Namur, Namur, Belgium. Electronic address: charles.nicaise@unamur.be.
Brain Res ; 1758: 147344, 2021 05 01.
Article in English | MEDLINE | ID: covidwho-1068877
ABSTRACT
Modelling cell infection in-a-dish can represent a useful tool to understand the susceptibility of different cell types towards severe acute respiratory coronavirus-2 (SARS-CoV-2) and to decipher its neurotropism. In this perspective, retinoic acid (RA)-differentiated neuroblastoma cell lines, SH-SY5Y and SK-N-BE(2) and glioblastoma cell lines, U-87 MG and U-373 MG, were infected with a SARS-CoV-2 strain, at various multiplicity-of-infection (MOI). We first demonstrated that the common entry genes - needed for invading epithelial cells - were expressed. RA-differentiation induced an upregulation of ace2 and tmprss2 gene expression while inducing downregulation of ctsb and ctsl. Using in situ hybridization and confocal analysis, SARS-CoV-2 gene S RNA was detected intracellularly at MOI 5.0, and localized in both soma and neuritic-like or glial-like processes. The infection was confirmed by quantification of viral gene E RNA and showed a dose-dependency, with few infected cells at MOI 0.1. After 24 h of infection, no cytopathic effect was observed in SH-SY5Y abilities to maintain neuritic processes or in U-373 MG for the uptake of glutamate. Unlike the permissive Vero E6 cells, no significant apoptosis death was detected following SARS-CoV-2 infection of neuroblastoma or glioblastoma cells. This study demonstrates the susceptibility of neuronal- and glial-like cell lines towards SARS-CoV-2 infection at high MOIs. Once inside the cells, the virus does not seem to rapidly replicate nor exert major cytopathic effect. Overall, our results strengthen the idea that SARS-CoV-2 has a tropism for nervous cells that express commonly described entry genes.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Glioblastoma / SARS-CoV-2 / COVID-19 / Neuroblastoma Limits: Humans Language: English Journal: Brain Res Year: 2021 Document Type: Article

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Glioblastoma / SARS-CoV-2 / COVID-19 / Neuroblastoma Limits: Humans Language: English Journal: Brain Res Year: 2021 Document Type: Article