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SARS-CoV-2 Seroprevalence in Healthcare Workers of Kaunas Hospitals during the First Wave of the COVID-19 Pandemic.
Pereckaite, Laura; Dambrauskiene, Asta; Urboniene, Daiva; Sadauskas, Saulius; Petrikonis, Kestutis; Naudziunas, Albinas; Vitkauskiene, Astra.
  • Pereckaite L; Department of Laboratory Medicine, The Hospital of Lithuanian University of Health Sciences Kauno Klinikos, 50161 Kaunas, Lithuania.
  • Dambrauskiene A; Department of Laboratory Medicine, The Hospital of Lithuanian University of Health Sciences Kauno Klinikos, 50161 Kaunas, Lithuania.
  • Urboniene D; Department of Laboratory Medicine, The Hospital of Lithuanian University of Health Sciences Kauno Klinikos, 50161 Kaunas, Lithuania.
  • Sadauskas S; Department of Internal Medicine, Kaunas Hospital of Lithuanian University of Health Sciences, 47144 Kaunas, Lithuania.
  • Petrikonis K; Department of Neurology, The Hospital of Lithuanian University of Health Sciences Kauno Klinikos, 50161 Kaunas, Lithuania.
  • Naudziunas A; Department of Internal Medicine, Kaunas Hospital of Lithuanian University of Health Sciences, 47144 Kaunas, Lithuania.
  • Vitkauskiene A; Department of Laboratory Medicine, The Hospital of Lithuanian University of Health Sciences Kauno Klinikos, 50161 Kaunas, Lithuania.
Medicina (Kaunas) ; 57(2)2021 Feb 06.
Article in English | MEDLINE | ID: covidwho-1069846
ABSTRACT
Background and

objective:

Serologic testing is a useful additional method for the diagnosis of COVID-19. It is also used for population-based seroepidemiological studies. The objective of the study was to determine SARS-CoV-2 seroprevalence in healthcare workers of Kaunas hospitals and to compare two methods for specific SARS-CoV-2 antibody testing. Materials and

Methods:

A total of 432 healthcare workers in Kaunas hospitals were enrolled in this study. Each participant filled a questionnaire including questions about their demographics, contact with suspected or confirmed COVID-19, acute respiratory symptoms, and whether they contacted their general practitioner, could not come to work, or had to be hospitalized. Capillary blood was used to test for SARS-CoV-2 specific immunoglobulin G (IgG) and immunoglobulin M (IgM) a lateral flow immunoassay. Serum samples were used to test for specific IgG and IgA class immunoglobulins using semiquantitative enzyme-linked immunosorbent assay (ELISA) method.

Results:

24.77% of study participants had direct contact with a suspected or confirmed case of COVID-19. A total of 64.81% of studied individuals had at least one symptom representing acute respiratory infection, compatible with COVID-19. Lateral flow immunoassay detected SARS-CoV-2 specific IgG class immunoglobulins in 1.16% of the tested group. Fever, cough, dyspnea, nausea, diarrhea, headache, conjunctivitis, muscle pain, and loss of smell and taste predominated in the anti-SARS-CoV-2 IgG-positive group. Using ELISA, specific IgG were detected in 1.32% of the tested samples. Diarrhea, loss of appetite, and loss of smell and taste sensations were the most predominant symptoms in anti-SARS-CoV-2 IgG-positive group. The positive percent agreement of the two testing methods was 50%, and negative percent agreement was 99.66%.

Conclusions:

1.16% of tested healthcare workers of Kaunas hospitals were anti-SARS-CoV-2 IgG-positive. The negative percent agreement of the lateral flow immunoassay and ELISA exceeded 99%.
Subject(s)
Keywords

Full text: Available Collection: International databases Database: MEDLINE Main subject: Personnel, Hospital / Immunoglobulin G / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study Topics: Long Covid Limits: Adult / Aged / Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal subject: Medicine Year: 2021 Document Type: Article Affiliation country: Medicina57020148

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Personnel, Hospital / Immunoglobulin G / COVID-19 Serological Testing / SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Observational study Topics: Long Covid Limits: Adult / Aged / Female / Humans / Male / Middle aged Country/Region as subject: Europa Language: English Journal subject: Medicine Year: 2021 Document Type: Article Affiliation country: Medicina57020148