Sample pooling on the Cepheid Xpert® Xpress SARS-CoV-2 assay.
Diagn Microbiol Infect Dis
; 99(2): 115238, 2021 Feb.
Article
in English
| MEDLINE | ID: covidwho-1071247
ABSTRACT
The COVID-19 pandemic has placed unprecedented global demand on laboratory supplies required for testing. Sample pooling has been investigated by laboratories as a strategy to preserve testing capacity. We evaluate the performance of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay for testing samples in pools of 4 and 6. Clinical samples containing SARS-CoV-2, and confirmed negative clinical samples were used to create sample pools. Clinical samples had 'neat' Xpert® E gene cycle threshold values ranging between 20 and 28 and all were detected qualitatively when contained in pools of 4 or 6 samples. For these samples, pooling had a median change in cycle threshold value of 2.0 in pools of 4, and of 2.9 in pools of 6. With the use of Cepheid Xpert® Xpress SARS-CoV-2 RT-PCR assay, pooling of 4 or 6 samples may be an effective strategy to increase testing capacity.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Clinical Laboratory Techniques
/
Reverse Transcriptase Polymerase Chain Reaction
/
Molecular Diagnostic Techniques
/
COVID-19 Testing
/
COVID-19
Type of study:
Diagnostic study
/
Experimental Studies
/
Prognostic study
/
Qualitative research
Limits:
Humans
Language:
English
Journal:
Diagn Microbiol Infect Dis
Year:
2021
Document Type:
Article
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