Your browser doesn't support javascript.
Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious Bronchitis Virus.
Zhang, Yu; Yuan, Yuan; Zhang, Li-Hua; Zhu, Dan; Wang, Lu; Wei, Lan-Ping; Fan, Wen-Sheng; Zhao, Chang-Run; Su, Yan-Jing; Liao, Jian-Qi; Yong, Lu; Wei, Tian-Chao; Wei, Ping; Mo, Mei-Lan.
  • Zhang Y; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Yuan Y; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Zhang LH; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Zhu D; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Wang L; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Wei LP; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Fan WS; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Zhao CR; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Su YJ; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Liao JQ; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Yong L; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Wei TC; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Wei P; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
  • Mo ML; College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
Vaccines (Basel) ; 9(2)2021 Feb 11.
Article in English | MEDLINE | ID: covidwho-1077468
Semantic information from SemMedBD (by NLM)
1. Structural protein PART_OF Virus-like particles
Subject
Structural protein
Predicate
PART_OF
Object
Virus-like particles
2. Structural protein PART_OF Infectious bronchitis virus
Subject
Structural protein
Predicate
PART_OF
Object
Infectious bronchitis virus
3. e protein compared_with M Protei
Subject
e protein
Predicate
compared_with
Object
M Protei
4. Vaccine AUGMENTS C0333785
Subject
Vaccine
Predicate
AUGMENTS
Object
C0333785
5. Vaccines AUGMENTS Virus-like particles
Subject
Vaccines
Predicate
AUGMENTS
Object
Virus-like particles
6. Kidney LOCATION_OF Vaccines
Subject
Kidney
Predicate
LOCATION_OF
Object
Vaccines
7. Kidney LOCATION_OF SMO wt Allele
Subject
Kidney
Predicate
LOCATION_OF
Object
SMO wt Allele
8. Tears body substance LOCATION_OF Vaccines
Subject
Tears body substance
Predicate
LOCATION_OF
Object
Vaccines
9. Tears body substance LOCATION_OF SMO wt Allele
Subject
Tears body substance
Predicate
LOCATION_OF
Object
SMO wt Allele
10. e protein PART_OF Infectious bronchitis virus
Subject
e protein
Predicate
PART_OF
Object
Infectious bronchitis virus
11. e protein STIMULATES Cells
Subject
e protein
Predicate
STIMULATES
Object
Cells
12. Structural protein PART_OF Virus-like particles
Subject
Structural protein
Predicate
PART_OF
Object
Virus-like particles
13. Structural protein PART_OF Infectious bronchitis virus
Subject
Structural protein
Predicate
PART_OF
Object
Infectious bronchitis virus
14. e protein compared_with M Protein, multiple myeloma
Subject
e protein
Predicate
compared_with
Object
M Protein, multiple myeloma
15. Vaccines, Inactivated AUGMENTS Virus-like particles
Subject
Vaccines, Inactivated
Predicate
AUGMENTS
Object
Virus-like particles
16. Vaccines AUGMENTS Virus-like particles
Subject
Vaccines
Predicate
AUGMENTS
Object
Virus-like particles
17. Kidney LOCATION_OF Vaccines
Subject
Kidney
Predicate
LOCATION_OF
Object
Vaccines
18. Kidney LOCATION_OF SMO wt Allele
Subject
Kidney
Predicate
LOCATION_OF
Object
SMO wt Allele
19. Tears body substance LOCATION_OF Vaccines
Subject
Tears body substance
Predicate
LOCATION_OF
Object
Vaccines
20. Tears body substance LOCATION_OF SMO wt Allele
Subject
Tears body substance
Predicate
LOCATION_OF
Object
SMO wt Allele
21. e protein PART_OF Infectious bronchitis virus
Subject
e protein
Predicate
PART_OF
Object
Infectious bronchitis virus
22. e protein STIMULATES Cells
Subject
e protein
Predicate
STIMULATES
Object
Cells
ABSTRACT
Infectious bronchitis virus (IBV) poses massive economic losses in the global poultry industry. Here, we firstly report the construction and immunogenicity comparison of virus-like particles (VLPs) carrying the S, M and E proteins (SME-VLPs); VLPs carrying the S and M proteins (SM-VLPs); and VLPs carrying the M and E proteins (ME-VLPs) from the dominant serotype representative strain GX-YL5 in China. The neutralizing antibody response induced by the SME-VLPs was similar to that induced by the inactivated oil vaccine (OEV) of GX-YL5, and higher than those induced by the SM-VLPs, ME-VLPs and commercial live vaccine H120. More importantly, the SME-VLPs elicited higher percentages of CD4+ and CD8+ T lymphocytes than the SM-VLPs, ME-VLPs and OEV of GX-YL5. Compared with the OEV of GX-YL5, higher levels of IL-4 and IFN-γ were also induced by the SME-VLPs. Moreover, the mucosal immune response (sIgA) induced by the SME-VLPs in the tear and oral swabs was comparable to that induced by the H120 vaccine and higher than that induced by the OEV of GX-YL5. In the challenge experiment, the SME-VLPs resulted in significantly lower viral RNA levels in the trachea and higher protection scores than the OEV of GX-YL5 and H120 vaccines, and induced comparable viral RNA levels in the kidneys, and tear and oral swabs to the OEV of GX-YL5. In summary, among the three VLPs, the SME-VLPs carrying the S, M and E proteins of IBV could stimulate the strongest humoral, cellular and mucosal immune responses and provide effective protection, indicating that it would be an attractive vaccine candidate for IB.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Year: 2021 Document Type: Article Affiliation country: Vaccines9020146

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Collection: International databases Database: MEDLINE Topics: Vaccines Language: English Year: 2021 Document Type: Article Affiliation country: Vaccines9020146