Sample pooling is efficient in PCR testing of SARS-CoV-2: a study in 7400 healthcare professionals.
Diagn Microbiol Infect Dis
; 100(1): 115330, 2021 May.
Article
in English
| MEDLINE | ID: covidwho-1077852
ABSTRACT
SARS-CoV-2 pandemic shows the importance of having efficient virus diagnosis, especially in groups of particular relevance such as health care professionals, without involving a large economic expense. This is a prevalence study carried out in 7400 health care professionals in a 1350-bed hospital in Madrid, Spain. Pools of 10 samples were performed, using the Xpert® Xpress SARS-CoV-2 test for the diagnosis from clinical samples of nasopharyngeal exudate. A previous study was performed to evaluate the effect of the dilution in terms of sensitivity. The estimated sensitivity was over 95%. A total of 740 pools were performed, with a final result of 218 health care professionals being positive. Using the pooling system, the reagent cost reduction to the institution was 75.3%. It can be concluded that the described sample pooling system is a useful and efficient tool in the diagnosis of SARS-CoV-2 in certain groups, assuming a cost reduction without reducing the sensitivity.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Personnel, Hospital
/
Polymerase Chain Reaction
/
COVID-19 Nucleic Acid Testing
Type of study:
Diagnostic study
/
Experimental Studies
/
Observational study
/
Prognostic study
/
Randomized controlled trials
Limits:
Humans
Country/Region as subject:
Europa
Language:
English
Journal:
Diagn Microbiol Infect Dis
Year:
2021
Document Type:
Article
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