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Morphometry of SARS-CoV and SARS-CoV-2 particles in ultrathin plastic sections of infected Vero cell cultures.
Laue, Michael; Kauter, Anne; Hoffmann, Tobias; Möller, Lars; Michel, Janine; Nitsche, Andreas.
  • Laue M; Advanced Light and Electron Microscopy, Centre for Biological Threats and Special Pathogens 4 (ZBS 4), Robert Koch Institute, Berlin, Germany. lauem@rki.de.
  • Kauter A; Advanced Light and Electron Microscopy, Centre for Biological Threats and Special Pathogens 4 (ZBS 4), Robert Koch Institute, Berlin, Germany.
  • Hoffmann T; Advanced Light and Electron Microscopy, Centre for Biological Threats and Special Pathogens 4 (ZBS 4), Robert Koch Institute, Berlin, Germany.
  • Möller L; Advanced Light and Electron Microscopy, Centre for Biological Threats and Special Pathogens 4 (ZBS 4), Robert Koch Institute, Berlin, Germany.
  • Michel J; Highly Pathogenic Viruses, Centre for Biological Threats and Special Pathogens 1 (ZBS 1), Robert Koch Institute, Berlin, Germany.
  • Nitsche A; Highly Pathogenic Viruses, Centre for Biological Threats and Special Pathogens 1 (ZBS 1), Robert Koch Institute, Berlin, Germany.
Sci Rep ; 11(1): 3515, 2021 02 10.
Article in English | MEDLINE | ID: covidwho-1078602
Preprint
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ABSTRACT
SARS-CoV-2 is the causative of the COVID-19 disease, which has spread pandemically around the globe within a few months. It is therefore necessary to collect fundamental information about the disease, its epidemiology and treatment, as well as about the virus itself. While the virus has been identified rapidly, detailed ultrastructural analysis of virus cell biology and architecture is still in its infancy. We therefore studied the virus morphology and morphometry of SARS-CoV-2 in comparison to SARS-CoV as it appears in Vero cell cultures by using conventional thin section electron microscopy and electron tomography. Both virus isolates, SARS-CoV Frankfurt 1 and SARS-CoV-2 Italy-INMI1, were virtually identical at the ultrastructural level and revealed a very similar particle size distribution with a median of about 100 nm without spikes. Maximal spike length of both viruses was 23 nm. The number of spikes per virus particle was about 30% higher in the SARS-CoV than in the SARS-CoV-2 isolate. This result complements a previous qualitative finding, which was related to a lower productivity of SARS-CoV-2 in cell culture in comparison to SARS-CoV.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virion / SARS-CoV-2 Type of study: Qualitative research Limits: Animals Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-82852-7

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Virion / SARS-CoV-2 Type of study: Qualitative research Limits: Animals Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-82852-7