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Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature.
Munitz, Ariel; Edry-Botzer, L; Itan, M; Tur-Kaspa, R; Dicker, D; Marcoviciu, D; Goren, M G; Mor, M; Lev, S; Gottesman, T; Muhsen, K; Cohen, D; Stein, M; Qimron, U; Freund, N T; Wine, Y; Gerlic, Motti.
  • Munitz A; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, 6997801, Ramat Aviv, Israel. arielm@post.tau.ac.il.
  • Edry-Botzer L; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, 6997801, Ramat Aviv, Israel.
  • Itan M; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, 6997801, Ramat Aviv, Israel.
  • Tur-Kaspa R; Department of Medicine D and the Liver Institute, Rabin Medical Center, Beilinson Hospital, Molecular Hepatology Research Laboratory, Felsenstein Medical Research Center Sackler School of Medicine Tel-Aviv University, Faculty of Medicine Bar-Ilan University, Liver Institute Rabin Medical Center Beil
  • Dicker D; Internal Medicine D, Hasharon Hospital-Rabin Medical Center, Petach Tikva, Israel.
  • Marcoviciu D; Sackler School of Medicine, Tel Aviv University, 6997801, Tel Aviv, Israel.
  • Goren MG; Internal Medicine D, Hasharon Hospital-Rabin Medical Center, Petach Tikva, Israel.
  • Mor M; Sackler School of Medicine, Tel Aviv University, 6997801, Tel Aviv, Israel.
  • Lev S; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, 6997801, Ramat Aviv, Israel.
  • Gottesman T; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, 6997801, Ramat Aviv, Israel.
  • Muhsen K; Sackler School of Medicine, Tel Aviv University, 6997801, Tel Aviv, Israel.
  • Cohen D; Intensive Care Unit, Hasharon Hospital-Rabin Medical Center, Petach Tikva, Israel.
  • Stein M; Sackler School of Medicine, Tel Aviv University, 6997801, Tel Aviv, Israel.
  • Qimron U; Infectious Diseases and Infection Control, Hasharon Hospital-Rabin Medical Center, Petach Tikva, Israel.
  • Freund NT; Department of Epidemiology and Preventive Medicine, School of Public Health, Tel Aviv University, 6997801, Tel Aviv, Israel.
  • Wine Y; Department of Epidemiology and Preventive Medicine, School of Public Health, Tel Aviv University, 6997801, Tel Aviv, Israel.
  • Gerlic M; Sackler School of Medicine, Tel Aviv University, 6997801, Tel Aviv, Israel.
Sci Rep ; 11(1): 3461, 2021 02 10.
Article in English | MEDLINE | ID: covidwho-1078603
Semantic information from SemMedBD (by NLM)
1. immunoglobulin G TREATS COVID-19
Subject
immunoglobulin G
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TREATS
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2. COVID-19 PROCESS_OF Patients
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immunoglobulin G
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4. Antigens PART_OF 2019 novel coronavirus
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5. Assay USES Electrochemiluminescence
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6. Borg Category-Ratio 10 Perceived Exertion Score 5 PROCESS_OF Patients
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7. Assay DIAGNOSES Rapid onset
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ABSTRACT
Despite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections.
Subject(s)

Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / Interleukin-12 / Interleukin-33 / Seroconversion / COVID-19 Type of study: Diagnostic study Topics: Long Covid Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-83019-0

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Immunoglobulin G / Interleukin-12 / Interleukin-33 / Seroconversion / COVID-19 Type of study: Diagnostic study Topics: Long Covid Limits: Humans Language: English Journal: Sci Rep Year: 2021 Document Type: Article Affiliation country: S41598-021-83019-0