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Berberine and Obatoclax Inhibit SARS-Cov-2 Replication in Primary Human Nasal Epithelial Cells In Vitro.
Varghese, Finny S; van Woudenbergh, Esther; Overheul, Gijs J; Eleveld, Marc J; Kurver, Lisa; van Heerbeek, Niels; van Laarhoven, Arjan; Miesen, Pascal; den Hartog, Gerco; de Jonge, Marien I; van Rij, Ronald P.
  • Varghese FS; Department of Medical Microbiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
  • van Woudenbergh E; Section Paediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
  • Overheul GJ; Centre for Immunology of Infectious Diseases and Vaccines, National Institute for Public Health and the Environment, 3721 MA Bilthoven, The Netherlands.
  • Eleveld MJ; Department of Medical Microbiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
  • Kurver L; Section Paediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
  • van Heerbeek N; Department of Internal Medicine, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
  • van Laarhoven A; Department of Otolaryngology, Head and Neck Surgery, Radboudumc, 6500 HB Nijmegen, The Netherlands.
  • Miesen P; Department of Internal Medicine, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
  • den Hartog G; Department of Medical Microbiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
  • de Jonge MI; Centre for Immunology of Infectious Diseases and Vaccines, National Institute for Public Health and the Environment, 3721 MA Bilthoven, The Netherlands.
  • van Rij RP; Section Paediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 6500 HB Nijmegen, The Netherlands.
Viruses ; 13(2)2021 02 11.
Article in English | MEDLINE | ID: covidwho-1079725
Preprint
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ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged as a new human pathogen in late 2019 and it has infected over 100 million people in less than a year. There is a clear need for effective antiviral drugs to complement current preventive measures, including vaccines. In this study, we demonstrate that berberine and obatoclax, two broad-spectrum antiviral compounds, are effective against multiple isolates of SARS-CoV-2. Berberine, a plant-derived alkaloid, inhibited SARS-CoV-2 at low micromolar concentrations and obatoclax, which was originally developed as an anti-apoptotic protein antagonist, was effective at sub-micromolar concentrations. Time-of-addition studies indicated that berberine acts on the late stage of the viral life cycle. In agreement, berberine mildly affected viral RNA synthesis, but it strongly reduced infectious viral titers, leading to an increase in the particle-to-pfu ratio. In contrast, obatoclax acted at the early stage of the infection, which is in line with its activity to neutralize the acidic environment in endosomes. We assessed infection of primary human nasal epithelial cells that were cultured on an air-liquid interface and found that SARS-CoV-2 infection induced and repressed expression of specific sets of cytokines and chemokines. Moreover, both obatoclax and berberine inhibited SARS-CoV-2 replication in these primary target cells. We propose berberine and obatoclax as potential antiviral drugs against SARS-CoV-2 that could be considered for further efficacy testing.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Pyrroles / Virus Replication / Berberine / SARS-CoV-2 / Indoles Topics: Vaccines Limits: Adolescent / Animals / Humans / Male Language: English Year: 2021 Document Type: Article Affiliation country: V13020282

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Antiviral Agents / Pyrroles / Virus Replication / Berberine / SARS-CoV-2 / Indoles Topics: Vaccines Limits: Adolescent / Animals / Humans / Male Language: English Year: 2021 Document Type: Article Affiliation country: V13020282