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Seroprevalence of neutralizing antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among healthcare workers in Makkah, Saudi Arabia.
Ahmed, Waleed A; Dada, Ashraf; Alshukairi, Abeer N; Sohrab, Sayed S; Faizo, Arwa A; Tolah, Ahmed M; El-Kafrawy, Sherif A; Bajrai, Leena H; Moalim, Hanan M; Aly, Mohamed H; Aboelazm, Ahmed F; Al-Hamzi, Mohammed A; Saeedi, Mohammed F; Alandijany, Thamir A; Azhar, Esam I.
  • Ahmed WA; Department of Medicine, Security Forces Hospital, Makkah, Saudi Arabia.
  • Dada A; Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital & Research Center, Jeddah, Saudi Arabia.
  • Alshukairi AN; Department of Medicine, King Faisal Specialist Hospital and Research Center, Jeddah, Saudi Arabia.
  • Sohrab SS; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Faizo AA; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Tolah AM; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
  • El-Kafrawy SA; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Bajrai LH; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Moalim HM; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Aly MH; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Aboelazm AF; Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Al-Hamzi MA; Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Saeedi MF; Department of Medicine, Security Forces Hospital, Makkah, Saudi Arabia.
  • Alandijany TA; Department of Medicine, Security Forces Hospital, Makkah, Saudi Arabia.
  • Azhar EI; Department of Infection Prevention and Control, Security Forces Hospital, Makkah, Saudi Arabia.
J King Saud Univ Sci ; 33(3): 101366, 2021 May.
Article in English | MEDLINE | ID: covidwho-1080393
Semantic information from SemMedBD (by NLM)
1. 2019 novel coronavirus PROCESS_OF Health Personnel
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
Object
Health Personnel
2. Problem PROCESS_OF Health Personnel
Subject
Problem
Predicate
PROCESS_OF
Object
Health Personnel
3. Problem PREDISPOSES COVID-19
Subject
Problem
Predicate
PREDISPOSES
Object
COVID-19
4. COVID-19 PROCESS_OF Health Personnel
Subject
COVID-19
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PROCESS_OF
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Health Personnel
5. Physicians DIAGNOSES COVID-19
Subject
Physicians
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DIAGNOSES
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COVID-19
6. Enzyme Immunoassay USES Chemiluminescence assay
Subject
Enzyme Immunoassay
Predicate
USES
Object
Chemiluminescence assay
7. Microneutralization Assay USES Chemiluminescence assay
Subject
Microneutralization Assay
Predicate
USES
Object
Chemiluminescence assay
8. Antibodies PART_OF 2019 novel coronavirus
Subject
Antibodies
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PART_OF
Object
2019 novel coronavirus
9. 2019 novel coronavirus PROCESS_OF Health Personnel
Subject
2019 novel coronavirus
Predicate
PROCESS_OF
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Health Personnel
10. Problem PROCESS_OF Health Personnel
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Problem
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PROCESS_OF
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Health Personnel
11. Problem PREDISPOSES COVID-19
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PREDISPOSES
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COVID-19
12. COVID-19 PROCESS_OF Health Personnel
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COVID-19
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PROCESS_OF
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Health Personnel
13. Physicians DIAGNOSES COVID-19
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Physicians
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DIAGNOSES
Object
COVID-19
14. Enzyme Immunoassay USES Chemiluminescence assay
Subject
Enzyme Immunoassay
Predicate
USES
Object
Chemiluminescence assay
15. Microneutralization Assay USES Chemiluminescence assay
Subject
Microneutralization Assay
Predicate
USES
Object
Chemiluminescence assay
16. Antibodies PART_OF 2019 novel coronavirus
Subject
Antibodies
Predicate
PART_OF
Object
2019 novel coronavirus
ABSTRACT

OBJECTIVE:

The new coronavirus disease 2019 (COVID-19) is a major health problem worldwide. The surveillance of seropositive individuals serves as an indicator to the extent of infection spread and provides an estimation of herd immunity status among population. Reports from different countries investigated this issue among healthcare workers (HCWs) who are "at risk" and "sources of risk" for COVID-19. This study aims to investigate the seroprevalence of COVID-19 among HCWs in one of the COVID-19 referral centers in Makkah, Saudi Arabia using three different serological methods.

METHODS:

In-house developed enzyme-linked immunoassay (ELISA), commercially available electro-chemiluminescence immunoassay (ECLIA), and microneutralization (MN) assay were utilized to determine the seroprevalence rate among the study population. 204 HCWs participated in the study. Both physicians and nurses working in the COVID-19 and non COVID-19 areas were included. Twelve out of 204 were confirmed cases of COVID-19 with variable disease severity. Samples from recovered HCWs were collected four weeks post diagnosis.

RESULTS:

The overall seroprevalence rate was 6.3% (13 out of 204) using the in-house ELISA and MN assay and it was 5.8% (12 out of 204) using the commercial ECLIA. Among HCWs undiagnosed with COVID-19, the seroprevalence was 2% (4 out 192). Notably, neutralizing antibodies were not detected in 3 (25%) out 12 confirmed cases of COVID-19.

CONCLUSIONS:

Our study, similar to the recent national multi-center study, showed a low seroprevalence of SARS-Cov-2 antibodies among HCWs. Concordance of results between the commercial electro-chemiluminescence immunoassay (ECLIA), in-house ELISA and MN assay was observed. The in-house ELISA is a promising tool for the serological diagnosis of SARS-CoV-2 infection. However, seroprevalence studies may underestimate the extent of COVID-19 infection as some cases with mild disease did not have detectable antibody responses.
Keywords

Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: J King Saud Univ Sci Year: 2021 Document Type: Article Affiliation country: J.jksus.2021.101366

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Full text: Available Collection: International databases Database: MEDLINE Language: English Journal: J King Saud Univ Sci Year: 2021 Document Type: Article Affiliation country: J.jksus.2021.101366